Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4805114..4805611 Replicon chromosome
Accession NZ_AP024112
Organism Escherichia coli strain ECSC054

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FE985_RS22945 Protein ID WP_001173343.1
Coordinates 4805114..4805287 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FE985_RS22950 Protein ID WP_000398619.1
Coordinates 4805315..4805611 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE985_RS22935 (4801784) 4801784..4803241 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
FE985_RS22940 (4803478) 4803478..4804995 + 1518 WP_001550472.1 lysine--tRNA ligase -
FE985_RS22945 (4805114) 4805114..4805287 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FE985_RS22950 (4805315) 4805315..4805611 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FE985_RS22955 (4805840) 4805840..4806112 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
FE985_RS22960 (4806124) 4806124..4806354 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
FE985_RS22965 (4806534) 4806534..4808165 + 1632 WP_001216477.1 sensor histidine kinase -
FE985_RS22970 (4808162) 4808162..4808881 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4747420..4805611 58191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T37890 WP_001173343.1 NZ_AP024112:c4805287-4805114 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T37890 NZ_AP024112:c4805287-4805114 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT37890 WP_000398619.1 NZ_AP024112:c4805611-4805315 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT37890 NZ_AP024112:c4805611-4805315 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References