Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3865846..3866464 Replicon chromosome
Accession NZ_AP024112
Organism Escherichia coli strain ECSC054

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FE985_RS18345 Protein ID WP_001291435.1
Coordinates 3866246..3866464 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FE985_RS18340 Protein ID WP_000344800.1
Coordinates 3865846..3866220 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE985_RS18330 (3860935) 3860935..3862128 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FE985_RS18335 (3862151) 3862151..3865300 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
FE985_RS18340 (3865846) 3865846..3866220 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FE985_RS18345 (3866246) 3866246..3866464 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FE985_RS18350 (3866636) 3866636..3867187 + 552 WP_000102564.1 maltose O-acetyltransferase -
FE985_RS18355 (3867303) 3867303..3867773 + 471 WP_000136192.1 YlaC family protein -
FE985_RS18360 (3867937) 3867937..3869487 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FE985_RS18365 (3869529) 3869529..3869882 - 354 WP_001550741.1 DUF1428 family protein -
FE985_RS18375 (3870261) 3870261..3870572 + 312 WP_000409911.1 MGMT family protein -
FE985_RS18380 (3870603) 3870603..3871175 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37884 WP_001291435.1 NZ_AP024112:3866246-3866464 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T37884 NZ_AP024112:3866246-3866464 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT37884 WP_000344800.1 NZ_AP024112:3865846-3866220 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT37884 NZ_AP024112:3865846-3866220 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References