Detailed information of TA system TA037863

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 72581..73415
• Replicon: chromosome
• Accession: NZ_AP024112
• Organism: Escherichia coli strain ECSC054

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A4S3WCD6
• Locus tag: FE985_RS00310
• Protein ID: WP_021566610.1
• Coordinates: 72581..72958 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FE985_RS00315
• Protein ID: WP_175117197.1
• Coordinates: 73047..73415 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE985_RS00280 (67948)	67948..68871	-	924	WP_000535949.1	carboxylate/amino acid/amine transporter	-
FE985_RS00285 (68982)	68982..70166	-	1185	WP_001172876.1	sugar efflux transporter	-
FE985_RS00290 (70571)	70571..70776	-	206	Protein_57	virulence RhuM family protein	-
FE985_RS00295 (70957)	70957..71802	-	846	WP_021566612.1	DUF4942 domain-containing protein	-
FE985_RS00300 (71887)	71887..72084	-	198	WP_000839269.1	DUF957 domain-containing protein	-
FE985_RS00305 (72096)	72096..72584	-	489	WP_048237286.1	DUF5983 family protein	-
FE985_RS00310 (72581)	72581..72958	-	378	WP_021566610.1	TA system toxin CbtA family protein	Toxin
FE985_RS00315 (73047)	73047..73415	-	369	WP_175117197.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
FE985_RS00320 (73489)	73489..73710	-	222	WP_000692323.1	DUF987 domain-containing protein	-
FE985_RS00325 (73773)	73773..74249	-	477	WP_001186726.1	RadC family protein	-
FE985_RS00330 (74265)	74265..74744	-	480	WP_048265132.1	antirestriction protein	-
FE985_RS00335 (74826)	74826..75644	-	819	WP_001234629.1	DUF932 domain-containing protein	-
FE985_RS00340 (75744)	75744..75977	-	234	WP_001119729.1	DUF905 family protein	-
FE985_RS00345 (76056)	76056..76510	-	455	Protein_68	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	61259..74744	13485

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.02 Da
• Isoelectric Point: 7.8276

>T37863 WP_021566610.1 NZ_AP024112:c72958-72581 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T37863 NZ_AP024112:c72958-72581 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13524.37 Da
• Isoelectric Point: 5.9505

>AT37863 WP_175117197.1 NZ_AP024112:c73415-73047 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADSAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT37863 NZ_AP024112:c73415-73047 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACAGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A4S3WCD6