Detailed information of TA system TA037857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 85695..86305
• Replicon: plasmid pMAFF241648
• Accession: NZ_AP024108
• Organism: Ralstonia solanacearum strain MAFF 241648

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0K1ZQG6
• Locus tag: JK148_RS18160
• Protein ID: WP_028853939.1
• Coordinates: 85997..86305 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JK148_RS18155
• Protein ID: WP_016722339.1
• Coordinates: 85695..86000 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK148_RS18130	82013..82294	-	282	WP_019719760.1	hypothetical protein	-
JK148_RS18135	82425..82709	-	285	WP_011003391.1	hypothetical protein	-
JK148_RS18140	83014..83231	+	218	Protein_70	hypothetical protein	-
JK148_RS18145	83398..84063	+	666	WP_197368577.1	hypothetical protein	-
JK148_RS18150	84169..85482	-	1314	WP_200763548.1	metabolite/H+ symporter	-
JK148_RS18155	85695..86000	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
JK148_RS18160	85997..86305	-	309	WP_028853939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JK148_RS18165	86381..87548	-	1168	Protein_75	MFS transporter	-
JK148_RS18170	87675..88496	-	822	WP_011003398.1	GntR family transcriptional regulator	-
JK148_RS18175	88674..89225	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
JK148_RS18180	89358..89957	+	600	WP_011003400.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / adeG / acrB / fliI / fliG / fliM / flgG / flgC / cyaB	1..2107950	2107950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11841.61 Da
• Isoelectric Point: 7.1367

>T37857 WP_028853939.1 NZ_AP024108:c86305-85997 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGNPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T37857 NZ_AP024108:c86305-85997 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCAACCCGGGCGACATGAAAGCGGTCCGTGACGGCATCCGTGAACTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT37857 WP_016722339.1 NZ_AP024108:c86000-85695 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT37857 NZ_AP024108:c86000-85695 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACCGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTCGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A0K1ZQG6