Detailed information of TA system TA037854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 480219..480852
• Replicon: chromosome
• Accession: NZ_AP024107
• Organism: Ralstonia solanacearum strain MAFF 241648

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JK148_RS02190
• Protein ID: WP_197369056.1
• Coordinates: 480439..480852 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8XUX7
• Locus tag: JK148_RS02185
• Protein ID: WP_011002949.1
• Coordinates: 480219..480452 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK148_RS02170	475807..477102	-	1296	WP_011002952.1	MFS transporter	-
JK148_RS02175	477110..478360	-	1251	WP_200763332.1	AGE family epimerase/isomerase	-
JK148_RS02180	478555..480162	+	1608	WP_020832850.1	glucan biosynthesis protein	-
JK148_RS02185	480219..480452	+	234	WP_011002949.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JK148_RS02190	480439..480852	+	414	WP_197369056.1	PIN domain-containing protein	Toxin
JK148_RS02195	480827..481453	-	627	WP_016725173.1	DUF1415 domain-containing protein	-
JK148_RS02200	481644..482015	+	372	WP_165591128.1	hypothetical protein	-
JK148_RS02205	482043..483263	-	1221	WP_011002488.1	ISL3-like element ISRso15 family transposase	-
JK148_RS02210	483397..485349	-	1953	WP_197369209.1	lytic transglycosylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	482043..483263	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15117.51 Da
• Isoelectric Point: 5.5348

>T37854 WP_197369056.1 NZ_AP024107:480439-480852 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIEMVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Nucleotide

• Length: 414 bp

>T37854 NZ_AP024107:480439-480852 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAATGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8790.98 Da
• Isoelectric Point: 8.7287

>AT37854 WP_011002949.1 NZ_AP024107:480219-480452 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT37854 NZ_AP024107:480219-480452 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A223GR76