Detailed information of TA system TA037850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 262209..262870
• Replicon: chromosome
• Accession: NZ_AP024105
• Organism: Ralstonia solanacearum strain MAFF 241647

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: JK149_RS01225
• Protein ID: WP_016727753.1
• Coordinates: 262209..262622 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: JK149_RS01230
• Protein ID: WP_019717749.1
• Coordinates: 262619..262870 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK149_RS01190	257531..257818	-	288	WP_086706365.1	hypothetical protein	-
JK149_RS01195	257771..258334	+	564	Protein_237	hypothetical protein	-
JK149_RS01200	258343..258495	+	153	WP_157698851.1	hypothetical protein	-
JK149_RS01205	258571..259215	+	645	WP_086706230.1	hypothetical protein	-
JK149_RS01210	259449..260294	-	846	WP_157698852.1	hypothetical protein	-
JK149_RS01215	260682..260975	-	294	WP_020371973.1	hypothetical protein	-
JK149_RS01220	261115..262137	+	1023	WP_086706232.1	DNA cytosine methyltransferase	-
JK149_RS01225	262209..262622	-	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
JK149_RS01230	262619..262870	-	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JK149_RS01235	263247..263479	+	233	Protein_245	hypothetical protein	-
JK149_RS01240	263633..263848	-	216	WP_071615547.1	hypothetical protein	-
JK149_RS01245	264122..264460	+	339	WP_016727784.1	glycine zipper 2TM domain-containing protein	-
JK149_RS01250	265135..265851	-	717	WP_049800424.1	Crp/Fnr family transcriptional regulator	-
JK149_RS01255	266552..267166	+	615	WP_157698853.1	hypothetical protein	-
JK149_RS01260	267227..267376	-	150	WP_155738951.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	248690..268338	19648
	-	flank	IS/Tn	-	-	267373..268338	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T37850 WP_016727753.1 NZ_AP024105:c262622-262209 [Ralstonia solanacearum]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T37850 NZ_AP024105:c262622-262209 [Ralstonia solanacearum]
TTGAGTTACCTGATCGACACCAATGTCCTGTCCGAATTGCGCCGCAAAGCGCCCGATGCGCGCGTGGTGGCCTGGATGCA
GGACCGCCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAGATTCGCAAAGGTATCGAGCGGCTGGACGACG
CGGCGCGCCGCCAGCACCTGATTGACTGGCTCGAAGTGGAACTGCCGAACTACTTTCTCGGCCGATTGCTCGACATCGAT
GCGCATACCGCCGACCGCTGGGGCCGGCTGATGTCCTCGGCCGGCCGCCCCCTGCCCGCCATCGACGGCCTGCTGGCCGC
GACCGCCCTGCAACACGACCTCACGCTGGTCACGCGCAACACCAAGGATTTCGCGGGGCTCGACGTCCAGCTCATCAACC
CATGGGAAGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT37850 WP_019717749.1 NZ_AP024105:c262870-262619 [Ralstonia solanacearum]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT37850 NZ_AP024105:c262870-262619 [Ralstonia solanacearum]
ATGCAAAGCTGGCAAATGCAGGCCGCCAAGGCGCGGTTTTCCGATGTCGTGAAGCGGGCGGCAGACGATGGCCCGCAGGA
AATCACCGTGCATGGCCGCCCGGTGGCGGTGGTGATCTCGCGCGCACTGTTCGATCGCTTGAGCGGCGGCGGTGAGTCGC
TGGTCAGCTTCATGCGCCAATCCCCGCTGGCCGACCAGGACGATGTCGTCTTTGAGCGCGAGCGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA