Detailed information of TA system TA037848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 49540..50150
• Replicon: plasmid pMAFF301560
• Accession: NZ_AP024104
• Organism: Ralstonia solanacearum strain MAFF 301560

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A454TTM1
• Locus tag: JK146_RS18165
• Protein ID: WP_058907722.1
• Coordinates: 49842..50150 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JK146_RS18160
• Protein ID: WP_016722339.1
• Coordinates: 49540..49845 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK146_RS18130	45038..45523	+	486	WP_016726511.1	hypothetical protein	-
JK146_RS18135	45854..46135	-	282	WP_058907724.1	hypothetical protein	-
JK146_RS18140	46267..46551	-	285	WP_011003391.1	hypothetical protein	-
JK146_RS18145	46856..47073	+	218	Protein_44	transposase	-
JK146_RS18150	47240..47908	+	669	WP_071507362.1	hypothetical protein	-
JK146_RS18155	48014..49327	-	1314	WP_071507363.1	metabolite/H+ symporter	-
JK146_RS18160	49540..49845	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
JK146_RS18165	49842..50150	-	309	WP_058907722.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JK146_RS18170	50226..51425	-	1200	WP_058907721.1	MFS transporter	-
JK146_RS18175	51552..52373	-	822	WP_011003398.1	GntR family transcriptional regulator	-
JK146_RS18180	52551..53102	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
JK146_RS18185	53235..53834	+	600	WP_071507364.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1936579	1936579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11863.66 Da
• Isoelectric Point: 7.8930

>T37848 WP_058907722.1 NZ_AP024104:c50150-49842 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T37848 NZ_AP024104:c50150-49842 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCCACCCAGGCAACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT37848 WP_016722339.1 NZ_AP024104:c49845-49540 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT37848 NZ_AP024104:c49845-49540 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACAGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A454TTM1