Detailed information of TA system TA037838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 85658..86268
• Replicon: plasmid pMAFF211479
• Accession: NZ_AP024100
• Organism: Ralstonia solanacearum strain MAFF 211479

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0K1ZQG6
• Locus tag: JK150_RS18110
• Protein ID: WP_028853939.1
• Coordinates: 85960..86268 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JK150_RS18105
• Protein ID: WP_016722339.1
• Coordinates: 85658..85963 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK150_RS18080	81976..82257	-	282	WP_019719760.1	hypothetical protein	-
JK150_RS18085	82388..82672	-	285	WP_011003391.1	hypothetical protein	-
JK150_RS18090	82977..83194	+	218	Protein_70	hypothetical protein	-
JK150_RS18095	83361..84026	+	666	WP_197368577.1	hypothetical protein	-
JK150_RS18100	84132..85445	-	1314	WP_071507363.1	metabolite/H+ symporter	-
JK150_RS18105	85658..85963	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
JK150_RS18110	85960..86268	-	309	WP_028853939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JK150_RS18115	86344..87511	-	1168	Protein_75	MFS transporter	-
JK150_RS18120	87638..88459	-	822	WP_011003398.1	GntR family transcriptional regulator	-
JK150_RS18125	88637..89188	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
JK150_RS18130	89321..89920	+	600	WP_011003400.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / adeG / acrB / fliI / fliG / fliM / flgG / flgC / cyaB	1..2131108	2131108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11841.61 Da
• Isoelectric Point: 7.1367

>T37838 WP_028853939.1 NZ_AP024100:c86268-85960 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGNPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T37838 NZ_AP024100:c86268-85960 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCAACCCGGGCGACATGAAAGCGGTCCGTGACGGCATCCGTGAACTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT37838 WP_016722339.1 NZ_AP024100:c85963-85658 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT37838 NZ_AP024100:c85963-85658 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACCGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTCGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A0K1ZQG6