Detailed information of TA system TA037829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 311326..312140
• Replicon: plasmid pVNTG1
• Accession: NZ_AP024089
• Organism: Vibrio nigripulchritudo strain TUMSAT-TG-2018

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VNTUMSATTG_RS29820
• Protein ID: WP_200768880.1
• Coordinates: 311326..311874 (-)
• Length: 183 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VNTUMSATTG_RS29825
• Protein ID: WP_200768881.1
• Coordinates: 311871..312140 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VNTUMSATTG_RS29795	307103..307270	-	168	WP_200768877.1	hypothetical protein	-
VNTUMSATTG_RS29800	307500..309262	-	1763	Protein_281	SgrR family transcriptional regulator	-
VNTUMSATTG_RS29805	309715..310149	+	435	Protein_282	GrpB family protein	-
VNTUMSATTG_RS29810	310548..311048	+	501	WP_200768878.1	hypothetical protein	-
VNTUMSATTG_RS29815	311131..311286	-	156	WP_200768879.1	hypothetical protein	-
VNTUMSATTG_RS29820	311326..311874	-	549	WP_200768880.1	GNAT family N-acetyltransferase	Toxin
VNTUMSATTG_RS29825	311871..312140	-	270	WP_200768881.1	DUF1778 domain-containing protein	Antitoxin
VNTUMSATTG_RS29830	312539..312724	+	186	WP_200769027.1	hypothetical protein	-
VNTUMSATTG_RS29835	312736..313077	+	342	WP_200768882.1	endonuclease	-
VNTUMSATTG_RS29840	313496..314212	-	717	WP_022614092.1	RES domain-containing protein	-
VNTUMSATTG_RS29845	314209..315174	-	966	WP_052189099.1	hypothetical protein	-
VNTUMSATTG_RS29850	315475..315783	-	309	WP_200768883.1	MepB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..351712	351712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-81)

Sequences

Toxin

Protein

• Length: 183 a.a.
• Molecular weight: 20627.94 Da
• Isoelectric Point: 9.1711

>T37829 WP_200768880.1 NZ_AP024089:c311874-311326 [Vibrio nigripulchritudo]
VSCGKEFVELSKSYHDRNSFDCGEQELNTFIKTQAAKHMEAGISRTMILPSTQPLLNQKIAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFQGSGLGKVSLVRALKYLWEVSHHMRAYAIVVDCLTDSAQAFYAKFGFEVLCEHNG
RIRMFLPMKTVERLFNQRENNN

Nucleotide

• Length: 549 bp

>T37829 NZ_AP024089:c311874-311326 [Vibrio nigripulchritudo]
GTGAGTTGTGGTAAAGAGTTTGTAGAACTTAGTAAATCATATCACGACCGAAATTCATTCGATTGTGGTGAGCAAGAGCT
AAATACATTCATCAAAACTCAAGCAGCAAAGCATATGGAAGCAGGTATTAGTCGAACAATGATTCTTCCTAGTACTCAGC
CATTGCTTAATCAGAAGATTGCAATATGTGCATTTTACAGTGTTGCGCCTAGTTCAATCAGTCGAGAAACCTTACCAGCG
CAACTAGCAAAGAAACTTCCTCGATACCCCATTCCAGTGTTTCTTTTAGCTCAGTTAGCTGTGCATAAGGAATTTCAGGG
CTCTGGGCTAGGAAAAGTCAGTTTAGTCCGTGCACTAAAGTACCTTTGGGAAGTTAGCCACCATATGAGGGCGTATGCTA
TTGTCGTGGACTGTCTGACTGATTCAGCACAGGCGTTTTATGCAAAATTTGGCTTTGAAGTATTGTGTGAACATAATGGA
CGCATTCGCATGTTTCTACCAATGAAAACAGTAGAAAGACTTTTTAACCAACGAGAAAATAATAATTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9796.17 Da
• Isoelectric Point: 4.4798

>AT37829 WP_200768881.1 NZ_AP024089:c312140-311871 [Vibrio nigripulchritudo]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATHVIEEHESIVVKDSAFDEFMAACDKAKAPNQALLEAA
KFTDESGIK

Nucleotide

• Length: 270 bp

>AT37829 NZ_AP024089:c312140-311871 [Vibrio nigripulchritudo]
ATGGCAACAGCACGTTTAGATATCCGCCTTGATGAAGAGATAAAAGCGAAAGCTGAAAAGGCATCAGCATTACTTGGTCT
GAAAAGTTTAACTGAATATGTAGTTAGGCTTATGGACGAAGATGCAACACACGTAATTGAAGAGCACGAAAGTATCGTGG
TTAAGGATAGTGCCTTTGACGAATTTATGGCGGCTTGTGACAAAGCAAAAGCGCCTAATCAAGCTTTGTTGGAAGCTGCA
AAATTCACAGATGAGAGCGGTATCAAGTGA