Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 128610..129346 Replicon plasmid pM941-a
Accession NZ_AP023455
Organism Klebsiella pneumoniae strain M941

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JLF44_RS27630 Protein ID WP_003026803.1
Coordinates 128864..129346 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JLF44_RS27625 Protein ID WP_003026799.1
Coordinates 128610..128876 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF44_RS27600 123701..124048 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
JLF44_RS27605 124097..125635 + 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
JLF44_RS27610 126534..126842 - 309 WP_017896554.1 hypothetical protein -
JLF44_RS27615 127336..127884 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
JLF44_RS27620 127931..128365 + 435 WP_001567367.1 cell envelope integrity protein TolA -
JLF44_RS27625 128610..128876 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JLF44_RS27630 128864..129346 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JLF44_RS27635 129547..130950 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JLF44_RS27640 130979..131611 - 633 WP_001567369.1 hypothetical protein -
JLF44_RS27645 131825..132031 + 207 Protein_135 helix-turn-helix domain-containing protein -
JLF44_RS27650 132229..133992 + 1764 WP_004200842.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / tet(A) - 1..185769 185769
- inside IScluster/Tn - - 123299..130950 7651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T37822 WP_003026803.1 NZ_AP023455:128864-129346 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T37822 NZ_AP023455:128864-129346 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT37822 WP_003026799.1 NZ_AP023455:128610-128876 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT37822 NZ_AP023455:128610-128876 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References