Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3314574..3315199 Replicon chromosome
Accession NZ_AP023453
Organism Klebsiella pneumoniae strain M941

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JLF44_RS16325 Protein ID WP_002882817.1
Coordinates 3314574..3314957 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JLF44_RS16330 Protein ID WP_004150355.1
Coordinates 3314957..3315199 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF44_RS16310 3311940..3312842 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JLF44_RS16315 3312839..3313474 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JLF44_RS16320 3313471..3314400 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JLF44_RS16325 3314574..3314957 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JLF44_RS16330 3314957..3315199 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JLF44_RS16335 3315404..3316321 + 918 WP_002882812.1 alpha/beta hydrolase -
JLF44_RS16340 3316335..3317276 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JLF44_RS16345 3317321..3317758 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JLF44_RS16350 3317755..3318615 - 861 WP_002882807.1 virulence factor BrkB family protein -
JLF44_RS16355 3318609..3319208 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T37805 WP_002882817.1 NZ_AP023453:c3314957-3314574 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T37805 NZ_AP023453:c3314957-3314574 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT37805 WP_004150355.1 NZ_AP023453:c3315199-3314957 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT37805 NZ_AP023453:c3315199-3314957 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References