Detailed information of TA system TA037799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4748423..4749039
• Replicon: chromosome
• Accession: NZ_AP023452
• Organism: Citrobacter koseri strain MPUCK001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: MPUCK001_RS22395
• Protein ID: WP_012133919.1
• Coordinates: 4748423..4748797 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MPUCK001_RS22400
• Protein ID: WP_160880417.1
• Coordinates: 4748797..4749039 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPUCK001_RS22380	4745926..4746828	+	903	WP_012133916.1	formate dehydrogenase O subunit beta	-
MPUCK001_RS22385	4746825..4747460	+	636	WP_049011887.1	formate dehydrogenase cytochrome b556 subunit	-
MPUCK001_RS22390	4747457..4748386	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
MPUCK001_RS22395	4748423..4748797	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
MPUCK001_RS22400	4748797..4749039	-	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
MPUCK001_RS22405	4749275..4750216	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
MPUCK001_RS22410	4750261..4750698	-	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MPUCK001_RS22415	4750695..4751567	-	873	WP_012133924.1	virulence factor BrkB family protein	-
MPUCK001_RS22420	4751561..4752160	-	600	WP_104867942.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T37799 WP_012133919.1 NZ_AP023452:c4748797-4748423 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T37799 NZ_AP023452:c4748797-4748423 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAATACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTTATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTCGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT37799 WP_160880417.1 NZ_AP023452:c4749039-4748797 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT37799 NZ_AP023452:c4749039-4748797 [Citrobacter koseri]
ATGATGGCTGGAATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Toxin

• AlphaFold DB: A8AL49