Detailed information of TA system TA037786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1895866..1896478
• Replicon: chromosome
• Accession: NZ_AP023452
• Organism: Citrobacter koseri strain MPUCK001

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: MPUCK001_RS08840
• Protein ID: WP_012906750.1
• Coordinates: 1896299..1896478 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MPUCK001_RS08835
• Protein ID: WP_190286983.1
• Coordinates: 1895866..1896276 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPUCK001_RS08810	1891068..1891985	+	918	WP_190286979.1	baseplate J/gp47 family protein	-
MPUCK001_RS08815	1891978..1892541	+	564	WP_190286980.1	phage tail protein I	-
MPUCK001_RS08820	1892534..1893166	+	633	Protein_1727	phage tail protein	-
MPUCK001_RS08825	1894223..1894759	+	537	WP_190286981.1	tail fiber assembly protein	-
MPUCK001_RS08830	1894834..1895811	-	978	WP_190286982.1	glycosyltransferase	-
MPUCK001_RS08835	1895866..1896276	-	411	WP_190286983.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MPUCK001_RS08840	1896299..1896478	-	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
MPUCK001_RS08845	1896786..1897580	-	795	WP_190287004.1	glycosyl transferase	-
MPUCK001_RS08850	1897724..1897975	-	252	WP_190286984.1	DinI family protein	-
MPUCK001_RS08855	1898301..1898624	+	324	WP_012133087.1	heavy metal-binding domain-containing protein	-
MPUCK001_RS08860	1898621..1899451	+	831	WP_047457875.1	N-acetylmuramoyl-L-alanine amidase	-
MPUCK001_RS08865	1899744..1900367	+	624	WP_012133085.1	helix-turn-helix domain-containing protein	-
MPUCK001_RS08870	1900454..1900954	-	501	WP_104868863.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T37786 WP_012906750.1 NZ_AP023452:c1896478-1896299 [Citrobacter koseri]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T37786 NZ_AP023452:c1896478-1896299 [Citrobacter koseri]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAGAAAGGGCTGGTAACGGTGAAACATCCACAAAAGGACATACCGCTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTTTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14931.80 Da
• Isoelectric Point: 4.2460

>AT37786 WP_190286983.1 NZ_AP023452:c1896276-1895866 [Citrobacter koseri]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGEAVPEATSVENWLDDPDYADVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT37786 NZ_AP023452:c1896276-1895866 [Citrobacter koseri]
ATGTTATACCCCGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGTTTTTCTGG
CGGCGATAACTATCAGGATGCAATCGAGAGCGTACGTGAAGCAATCGACGCACATATAGAATTACTGGTGGAAGATGGTG
AAGCTGTACCGGAAGCTACCAGTGTGGAAAACTGGCTGGACGATCCTGATTACGCAGATGTAGTATGGGCGCTGGTGGAT
GTGGATGTTACGCGATTGATGGGGAAAGCTGAAAAAATCAATGTTACTCTTCCCTCTTTACTGATCCGACGTATCGACCA
GTTTGTCGCTGCACACCCTGAGTACGGTAGCCGTTCTGGCTTCCTGTCCCGCGTAGCTGCTGATAAAGTGATAGGACGAG
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4