Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1436114..1436734 Replicon chromosome
Accession NZ_AP023452
Organism Citrobacter koseri strain MPUCK001

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MPUCK001_RS06625 Protein ID WP_002892050.1
Coordinates 1436114..1436332 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag MPUCK001_RS06630 Protein ID WP_012133514.1
Coordinates 1436360..1436734 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPUCK001_RS06590 1431452..1432066 + 615 WP_104868983.1 hypothetical protein -
MPUCK001_RS06595 1432146..1432499 + 354 WP_012133521.1 DUF1428 family protein -
MPUCK001_RS06600 1432539..1434089 - 1551 WP_190286947.1 EAL domain-containing protein -
MPUCK001_RS06605 1434315..1434575 + 261 WP_012133519.1 type B 50S ribosomal protein L31 -
MPUCK001_RS06610 1434577..1434717 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MPUCK001_RS06615 1434795..1435265 - 471 WP_024130587.1 membrane protein -
MPUCK001_RS06620 1435379..1435930 - 552 WP_049009805.1 maltose O-acetyltransferase -
MPUCK001_RS06625 1436114..1436332 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
MPUCK001_RS06630 1436360..1436734 - 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
MPUCK001_RS06635 1437148..1438164 + 1017 WP_190286622.1 IS5-like element IS5 family transposase -
MPUCK001_RS06640 1438435..1441581 - 3147 WP_012133513.1 efflux RND transporter permease AcrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1437184..1438164 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37785 WP_002892050.1 NZ_AP023452:c1436332-1436114 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T37785 NZ_AP023452:c1436332-1436114 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT37785 WP_012133514.1 NZ_AP023452:c1436734-1436360 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT37785 NZ_AP023452:c1436734-1436360 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACAAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References