Detailed information of TA system TA037781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9107..9750
• Replicon: plasmid pN260-2
• Accession: NZ_AP023449
• Organism: Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A155IU92
• Locus tag: OIPHN260_RS25190
• Protein ID: WP_000754567.1
• Coordinates: 9334..9750 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A155IUD1
• Locus tag: OIPHN260_RS25185
• Protein ID: WP_023293777.1
• Coordinates: 9107..9337 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OIPHN260_RS25160	4545..5453	+	909	WP_001372261.1	HNH endonuclease	-
OIPHN260_RS25165	5665..6646	+	982	Protein_4	IS5-like element ISKpn26 family transposase	-
OIPHN260_RS25170	7042..7359	-	318	WP_047345246.1	DUF305 domain-containing protein	-
OIPHN260_RS25175	7431..8129	+	699	Protein_6	IS1 family transposase	-
OIPHN260_RS25180	8209..8553	+	345	WP_047747546.1	hypothetical protein	-
OIPHN260_RS25185	9107..9337	+	231	WP_023293777.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
OIPHN260_RS25190	9334..9750	+	417	WP_000754567.1	type II toxin-antitoxin system VapC family toxin	Toxin
OIPHN260_RS25195	10101..11069	-	969	WP_008502226.1	IS5-like element IS903B family transposase	-
OIPHN260_RS25200	11103..11240	-	138	Protein_11	sugar dehydrogenase	-
OIPHN260_RS25205	11306..12007	-	702	WP_008502228.1	DsbA family oxidoreductase	-
OIPHN260_RS25210	12073..13179	-	1107	WP_006687059.1	alkene reductase	-
OIPHN260_RS25215	13392..13721	+	330	WP_202324598.1	thioredoxin family protein	-
OIPHN260_RS25220	13751..14089	-	339	WP_000888080.1	carboxymuconolactone decarboxylase family protein	-
OIPHN260_RS25225	14094..14675	-	582	WP_008502230.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10	mrkA / mrkB / mrkC / mrkF / mrkJ	1..244996	244996
	-	inside	IScluster/Tn	-	-	3169..18829	15660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15108.61 Da
• Isoelectric Point: 8.5403

>T37781 WP_000754567.1 NZ_AP023449:9334-9750 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T37781 NZ_AP023449:9334-9750 [Enterobacter roggenkampii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.73 Da
• Isoelectric Point: 4.4299

>AT37781 WP_023293777.1 NZ_AP023449:9107-9337 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Nucleotide

• Length: 231 bp

>AT37781 NZ_AP023449:9107-9337 [Enterobacter roggenkampii]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: A0A155IU92

Antitoxin

• AlphaFold DB: A0A155IUD1