Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 164026..165649 Replicon plasmid pN260-1
Accession NZ_AP023448
Organism Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag OIPHN260_RS24635 Protein ID WP_000088044.1
Coordinates 164026..165351 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag OIPHN260_RS24640 Protein ID WP_012477377.1
Coordinates 165356..165649 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIPHN260_RS24615 161525..162529 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
OIPHN260_RS24620 162807..163235 - 429 Protein_183 hypothetical protein -
OIPHN260_RS24625 163248..163658 - 411 WP_000985909.1 hypothetical protein -
OIPHN260_RS24630 163759..163965 - 207 WP_000134171.1 hypothetical protein -
OIPHN260_RS24635 164026..165351 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
OIPHN260_RS24640 165356..165649 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
OIPHN260_RS24645 166050..167438 - 1389 WP_012695445.1 hypothetical protein -
OIPHN260_RS24650 167443..167940 - 498 WP_000340139.1 membrane protein -
OIPHN260_RS24655 168143..168799 - 657 WP_000462754.1 trypsin-like serine protease -
OIPHN260_RS24660 169458..169850 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-9 / blaCTX-M-9 / sul1 / aadA2 / dfrA16 / qnrA1 - 1..272108 272108


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T37780 WP_000088044.1 NZ_AP023448:c165351-164026 [Enterobacter roggenkampii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T37780 NZ_AP023448:c165351-164026 [Enterobacter roggenkampii]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT37780 WP_012477377.1 NZ_AP023448:c165649-165356 [Enterobacter roggenkampii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT37780 NZ_AP023448:c165649-165356 [Enterobacter roggenkampii]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References