Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1844499..1845296 Replicon chromosome
Accession NZ_AP023447
Organism Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)


Gene name KacT Uniprot ID A0A155TD66
Locus tag OIPHN260_RS09040 Protein ID WP_032651255.1
Coordinates 1844775..1845296 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W7PG43
Locus tag OIPHN260_RS09035 Protein ID WP_008499829.1
Coordinates 1844499..1844768 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIPHN260_RS09005 1840121..1840564 - 444 WP_047745123.1 helix-turn-helix transcriptional regulator -
OIPHN260_RS09010 1840717..1841958 + 1242 WP_023343731.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
OIPHN260_RS09015 1841994..1842221 - 228 WP_008499832.1 YccJ family protein -
OIPHN260_RS09020 1842242..1842838 - 597 WP_008499831.1 NAD(P)H:quinone oxidoreductase -
OIPHN260_RS09025 1843230..1843400 + 171 WP_013097201.1 general stress protein -
OIPHN260_RS09030 1843520..1844437 + 918 WP_202324496.1 DMT family transporter -
OIPHN260_RS09035 1844499..1844768 + 270 WP_008499829.1 DUF1778 domain-containing protein Antitoxin
OIPHN260_RS09040 1844775..1845296 + 522 WP_032651255.1 GNAT family N-acetyltransferase Toxin
OIPHN260_RS09045 1845370..1846692 - 1323 WP_008499826.1 pyrimidine utilization transport protein G -
OIPHN260_RS09050 1846714..1847208 - 495 WP_023292823.1 pyrimidine utilization flavin reductase protein F -
OIPHN260_RS09055 1847218..1847808 - 591 WP_047745125.1 malonic semialdehyde reductase -
OIPHN260_RS09060 1847818..1848618 - 801 WP_021240807.1 pyrimidine utilization protein D -
OIPHN260_RS09065 1848626..1849012 - 387 WP_047745126.1 pyrimidine utilization protein C -
OIPHN260_RS09070 1849024..1849713 - 690 WP_047745127.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19714.46 Da        Isoelectric Point: 7.0497

>T37769 WP_032651255.1 NZ_AP023447:1844775-1845296 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTDENSHSLF
FPTKSIERLFEQA

Download         Length: 522 bp

>T37769 NZ_AP023447:1844775-1845296 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGATGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10262.72 Da        Isoelectric Point: 5.8836

>AT37769 WP_008499829.1 NZ_AP023447:1844499-1844768 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT37769 NZ_AP023447:1844499-1844768 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCACCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155TD66

Antitoxin

Source ID Structure
AlphaFold DB W7PG43

References