Detailed information of TA system TA037766

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1013091..1013770
• Replicon: chromosome
• Accession: NZ_AP023447
• Organism: Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7U3LSF0
• Locus tag: OIPHN260_RS04980
• Protein ID: WP_023305529.1
• Coordinates: 1013091..1013432 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8H9RPW5
• Locus tag: OIPHN260_RS04985
• Protein ID: WP_001548170.1
• Coordinates: 1013453..1013770 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OIPHN260_RS04955	1008259..1008660	+	402	WP_008500249.1	sigma factor-binding protein Crl	-
OIPHN260_RS04960	1008797..1009849	-	1053	WP_008501878.1	phosphoporin PhoE	-
OIPHN260_RS04965	1010154..1011257	+	1104	WP_021242883.1	glutamate 5-kinase	-
OIPHN260_RS04970	1011269..1012522	+	1254	WP_023326745.1	glutamate-5-semialdehyde dehydrogenase	-
OIPHN260_RS04980	1013091..1013432	-	342	WP_023305529.1	TA system toxin CbtA family protein	Toxin
OIPHN260_RS04985	1013453..1013770	-	318	WP_001548170.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
OIPHN260_RS04990	1013790..1014011	-	222	WP_001548171.1	DUF987 domain-containing protein	-
OIPHN260_RS04995	1014020..1014496	-	477	WP_000811693.1	RadC family protein	-
OIPHN260_RS05000	1014512..1014970	-	459	WP_047746256.1	antirestriction protein	-
OIPHN260_RS05005	1015068..1015307	-	240	WP_000194654.1	DUF905 domain-containing protein	-
OIPHN260_RS05010	1015384..1015851	-	468	WP_001547765.1	hypothetical protein	-
OIPHN260_RS05015	1015880..1016317	-	438	WP_016154551.1	hypothetical protein	-
OIPHN260_RS05020	1016317..1016553	-	237	WP_001144031.1	hypothetical protein	-
OIPHN260_RS05025	1016594..1017295	-	702	WP_023305533.1	WYL domain-containing protein	-
OIPHN260_RS05030	1017513..1018334	-	822	WP_008324532.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1012871..1023276	10405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13003.05 Da
• Isoelectric Point: 10.2764

>T37766 WP_023305529.1 NZ_AP023447:c1013432-1013091 [Enterobacter roggenkampii]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T37766 NZ_AP023447:c1013432-1013091 [Enterobacter roggenkampii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11883.46 Da
• Isoelectric Point: 7.0810

>AT37766 WP_001548170.1 NZ_AP023447:c1013770-1013453 [Enterobacter roggenkampii]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT37766 NZ_AP023447:c1013770-1013453 [Enterobacter roggenkampii]
ATGACCCACATCAACTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCATATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A7U3LSF0