Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 637450..638026 Replicon chromosome
Accession NZ_AP023447
Organism Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag OIPHN260_RS03150 Protein ID WP_025912099.1
Coordinates 637450..637737 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag OIPHN260_RS03155 Protein ID WP_023293359.1
Coordinates 637724..638026 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIPHN260_RS03120 632461..632577 + 117 Protein_605 amino acid-binding protein -
OIPHN260_RS03125 632570..633265 + 696 WP_045371542.1 winged helix-turn-helix domain-containing protein -
OIPHN260_RS03130 633412..633882 + 471 WP_047746530.1 MarR family transcriptional regulator -
OIPHN260_RS03135 633879..634946 + 1068 WP_025912094.1 HlyD family secretion protein -
OIPHN260_RS03140 634936..636012 + 1077 WP_047746527.1 DUF2955 domain-containing protein -
OIPHN260_RS03145 636009..637280 - 1272 WP_025912097.1 DUF445 domain-containing protein -
OIPHN260_RS03150 637450..637737 + 288 WP_025912099.1 BrnT family toxin Toxin
OIPHN260_RS03155 637724..638026 + 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
OIPHN260_RS03160 638057..638695 - 639 WP_048962264.1 LysE family translocator -
OIPHN260_RS03165 638743..639486 - 744 WP_046092376.1 AraC family transcriptional regulator -
OIPHN260_RS03170 639638..641008 + 1371 WP_181518993.1 NAD-dependent succinate-semialdehyde dehydrogenase -
OIPHN260_RS03175 641053..641373 - 321 WP_008501314.1 hypothetical protein -
OIPHN260_RS03180 641373..641918 - 546 WP_047746519.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T37765 WP_025912099.1 NZ_AP023447:637450-637737 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T37765 NZ_AP023447:637450-637737 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTTTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT37765 WP_023293359.1 NZ_AP023447:637724-638026 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT37765 NZ_AP023447:637724-638026 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGAGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGTGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGATGCGGAGATTGATTACAGCGATATTCCTGCCACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References