Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 97824..98431 Replicon chromosome
Accession NZ_AP023447
Organism Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H0N5B9
Locus tag OIPHN260_RS00440 Protein ID WP_071993467.1
Coordinates 98246..98431 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A155E9Y3
Locus tag OIPHN260_RS00435 Protein ID WP_032675741.1
Coordinates 97824..98231 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OIPHN260_RS00405 93031..93264 - 234 WP_072065282.1 AlpA family transcriptional regulator -
OIPHN260_RS00410 93549..94187 + 639 WP_202324304.1 hypothetical protein -
OIPHN260_RS00415 94226..95208 - 983 Protein_81 IS5-like element ISKpn26 family transposase -
OIPHN260_RS00420 95430..95807 - 378 WP_202324306.1 helix-turn-helix domain-containing protein -
OIPHN260_RS00425 96045..96356 + 312 Protein_83 hypothetical protein -
OIPHN260_RS00430 96391..97407 + 1017 WP_001310555.1 IS5-like element IS5 family transposase -
OIPHN260_RS00435 97824..98231 - 408 WP_032675741.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OIPHN260_RS00440 98246..98431 - 186 WP_071993467.1 type II toxin-antitoxin system HicA family toxin Toxin
OIPHN260_RS00445 98641..99381 - 741 WP_021242443.1 MipA/OmpV family protein -
OIPHN260_RS00450 99499..100464 + 966 WP_063435980.1 LysR family transcriptional regulator -
OIPHN260_RS00455 100461..101999 - 1539 WP_023617402.1 aldehyde dehydrogenase AldB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6882.06 Da        Isoelectric Point: 11.2341

>T37763 WP_071993467.1 NZ_AP023447:c98431-98246 [Enterobacter roggenkampii]
VKSADVITVLISHGWKCVRTKGSHHQFRHPEQKGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T37763 NZ_AP023447:c98431-98246 [Enterobacter roggenkampii]
GTGAAAAGTGCGGATGTCATTACTGTTCTGATCAGCCATGGCTGGAAATGTGTCAGAACGAAAGGAAGCCATCATCAGTT
TCGCCATCCCGAACAGAAAGGGCTGGTTACTGTCCCACATCCCAAGAAGGATATTAAACCGGGCACTCTCGCCCAGATCT
GGCGACAGGCGGGTATTAAACACTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 15010.78 Da        Isoelectric Point: 4.3897

>AT37763 WP_032675741.1 NZ_AP023447:c98231-97824 [Enterobacter roggenkampii]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDNALPLPGNVEAHLESRPEDFTGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSFVSEHPQFGNRSAFLAEAARRVLPG

Download         Length: 408 bp

>AT37763 NZ_AP023447:c98231-97824 [Enterobacter roggenkampii]
ATGTTTTATCCGGCTTACATTCATTCTGACTCCGACGGTAGCGCCAGCGGCTTTTTCCCTGACGTTCCAGGCTGTTTTTT
TGCAGGTGATTCCCTGGACGACGCTTTTCAGGATGCGCGTGATGCGCTCACAGCACACTTCGAAGCGCTATTTGAAATGG
ATAATGCACTGCCTCTTCCGGGTAATGTGGAAGCGCATCTGGAAAGTCGTCCTGAGGATTTCACTGGCGGGCAATGGCTG
TTGGTAGATATTAATATGAAACAGTTCGACGGTAGGGCCGAGCGTATCAACATCACGATGCCTCGACGTTTGCTGGTAAA
AATTGATTCCTTTGTCAGCGAGCATCCTCAGTTCGGTAATCGAAGCGCATTTCTGGCTGAGGCAGCACGTCGCGTGCTGC
CTGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H0N5B9

Antitoxin

Source ID Structure
AlphaFold DB A0A155E9Y3

References