Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 85965..86566 Replicon plasmid pM71903
Accession NZ_AP023436
Organism Escherichia coli strain M719

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag M2043_RS25265 Protein ID WP_001216045.1
Coordinates 85965..86345 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag M2043_RS25270 Protein ID WP_001190712.1
Coordinates 86345..86566 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2043_RS25235 (MYEC719_p30104) 81410..81925 - 516 WP_248537801.1 hypothetical protein -
M2043_RS25240 (MYEC719_p30105) 81970..82851 - 882 WP_248537803.1 hypothetical protein -
M2043_RS25245 (MYEC719_p30106) 82893..84086 - 1194 WP_000219605.1 terminase -
M2043_RS25250 (MYEC719_p30107) 84225..84623 - 399 WP_248537805.1 hypothetical protein -
M2043_RS25255 (MYEC719_p30108) 84712..85650 - 939 WP_152924590.1 DUF968 domain-containing protein -
M2043_RS25260 (MYEC719_p30109) 85781..85960 - 180 WP_000113019.1 hypothetical protein -
M2043_RS25265 (MYEC719_p30110) 85965..86345 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
M2043_RS25270 (MYEC719_p30111) 86345..86566 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M2043_RS25275 (MYEC719_p30112) 86639..87028 - 390 WP_000506726.1 S24 family peptidase -
M2043_RS25280 (MYEC719_p30113) 87089..88309 - 1221 WP_000343760.1 ISL3-like element ISKox3 family transposase -
M2043_RS25285 (MYEC719_p30114) 88476..88727 - 252 WP_001377386.1 DNA polymerase III subunit theta -
M2043_RS25290 (MYEC719_p30116) 89177..89314 + 138 WP_000123562.1 hypothetical protein -
M2043_RS25295 (MYEC719_p30117) 89389..89751 - 363 WP_001261544.1 hypothetical protein -
M2043_RS25300 (MYEC719_p30118) 89748..90671 - 924 WP_176486947.1 hypothetical protein -
M2043_RS25305 (MYEC719_p30119) 90653..91030 - 378 WP_248537807.1 hypothetical protein -
M2043_RS25310 (MYEC719_p30120) 91037..91330 - 294 WP_001677496.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..118399 118399
- flank IS/Tn - - 87089..88309 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T37750 WP_001216045.1 NZ_AP023436:c86345-85965 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T37750 NZ_AP023436:c86345-85965 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT37750 WP_001190712.1 NZ_AP023436:c86566-86345 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT37750 NZ_AP023436:c86566-86345 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References