Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3957704..3958358 Replicon chromosome
Accession NZ_AP023433
Organism Escherichia coli strain M719

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag M2043_RS19065 Protein ID WP_000244777.1
Coordinates 3957704..3958111 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag M2043_RS19070 Protein ID WP_000354046.1
Coordinates 3958092..3958358 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2043_RS19045 (3953661) 3953661..3955394 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
M2043_RS19050 (3955400) 3955400..3956110 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M2043_RS19055 (3956135) 3956135..3957031 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
M2043_RS19060 (3957143) 3957143..3957664 + 522 WP_001055874.1 flavodoxin FldB -
M2043_RS19065 (3957704) 3957704..3958111 - 408 WP_000244777.1 protein YgfX Toxin
M2043_RS19070 (3958092) 3958092..3958358 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
M2043_RS19075 (3958601) 3958601..3959581 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
M2043_RS19080 (3959777) 3959777..3960436 - 660 WP_000250274.1 hemolysin III family protein -
M2043_RS19085 (3960600) 3960600..3960911 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
M2043_RS19090 (3960956) 3960956..3962389 + 1434 WP_001446971.1 6-phospho-beta-glucosidase BglA -
M2043_RS19095 (3962446) 3962446..3963189 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T37739 WP_000244777.1 NZ_AP023433:c3958111-3957704 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T37739 NZ_AP023433:c3958111-3957704 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT37739 WP_000354046.1 NZ_AP023433:c3958358-3958092 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT37739 NZ_AP023433:c3958358-3958092 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References