Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1352074..1352692 Replicon chromosome
Accession NZ_AP023433
Organism Escherichia coli strain M719

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M2043_RS06490 Protein ID WP_001291435.1
Coordinates 1352074..1352292 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M2043_RS06495 Protein ID WP_000344800.1
Coordinates 1352318..1352692 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2043_RS06455 (1347363) 1347363..1347935 + 573 WP_000779831.1 YbaY family lipoprotein -
M2043_RS06460 (1347966) 1347966..1348277 - 312 WP_000409911.1 MGMT family protein -
M2043_RS06470 (1348656) 1348656..1349009 + 354 WP_000878140.1 DUF1428 domain-containing protein -
M2043_RS06475 (1349051) 1349051..1350601 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M2043_RS06480 (1350765) 1350765..1351235 - 471 WP_000136192.1 YlaC family protein -
M2043_RS06485 (1351351) 1351351..1351902 - 552 WP_000102536.1 maltose O-acetyltransferase -
M2043_RS06490 (1352074) 1352074..1352292 - 219 WP_001291435.1 HHA domain-containing protein Toxin
M2043_RS06495 (1352318) 1352318..1352692 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M2043_RS06500 (1353238) 1353238..1356387 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
M2043_RS06505 (1356410) 1356410..1357603 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37726 WP_001291435.1 NZ_AP023433:c1352292-1352074 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T37726 NZ_AP023433:c1352292-1352074 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT37726 WP_000344800.1 NZ_AP023433:c1352692-1352318 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT37726 NZ_AP023433:c1352692-1352318 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References