Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 20063..20664 Replicon plasmid pHFQ1
Accession NZ_AP023428
Organism Escherichia coli strain HUE1

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag IF662_RS21905 Protein ID WP_001216034.1
Coordinates 20063..20443 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IF662_RS21910 Protein ID WP_001190712.1
Coordinates 20443..20664 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF662_RS21870 15886..16866 - 981 Protein_17 IS5-like element ISKpn26 family transposase -
IF662_RS21875 17054..17263 + 210 WP_000874156.1 hypothetical protein -
IF662_RS21880 17374..18225 + 852 WP_000611649.1 hypothetical protein -
IF662_RS21885 18258..18620 - 363 Protein_20 phage antirepressor KilAC domain-containing protein -
IF662_RS21890 18676..19373 + 698 Protein_21 IS1 family transposase -
IF662_RS21895 19387..19851 - 465 Protein_22 hypothetical protein -
IF662_RS21900 19879..20058 - 180 WP_038997082.1 hypothetical protein -
IF662_RS21905 20063..20443 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IF662_RS21910 20443..20664 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IF662_RS21915 20847..22403 + 1557 WP_053444764.1 type I restriction-modification system subunit M -
IF662_RS21920 22400..23638 + 1239 WP_053444758.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid floR / sul2 / aadA5 / qacE / sul1 / aac(3)-IIa / oqxB / oqxA - 1..120829 120829


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T37719 WP_001216034.1 NZ_AP023428:c20443-20063 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T37719 NZ_AP023428:c20443-20063 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT37719 WP_001190712.1 NZ_AP023428:c20664-20443 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT37719 NZ_AP023428:c20664-20443 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References