Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3395734..3396352 Replicon chromosome
Accession NZ_AP023427
Organism Escherichia coli strain HUE1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IF662_RS16270 Protein ID WP_001291435.1
Coordinates 3396134..3396352 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IF662_RS16265 Protein ID WP_000344800.1
Coordinates 3395734..3396108 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF662_RS16255 3390823..3392016 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IF662_RS16260 3392039..3395188 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IF662_RS16265 3395734..3396108 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IF662_RS16270 3396134..3396352 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IF662_RS16275 3396524..3397075 + 552 WP_000102564.1 maltose O-acetyltransferase -
IF662_RS16280 3397191..3397661 + 471 WP_000136192.1 YlaC family protein -
IF662_RS16285 3397825..3399375 + 1551 WP_063501968.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IF662_RS16290 3399417..3399770 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IF662_RS16300 3400149..3400460 + 312 WP_000409911.1 MGMT family protein -
IF662_RS16305 3400491..3401063 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37715 WP_001291435.1 NZ_AP023427:3396134-3396352 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T37715 NZ_AP023427:3396134-3396352 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT37715 WP_000344800.1 NZ_AP023427:3395734-3396108 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT37715 NZ_AP023427:3395734-3396108 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References