Detailed information of TA system TA037713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2426956..2427594
• Replicon: chromosome
• Accession: NZ_AP023427
• Organism: Escherichia coli strain HUE1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IF662_RS11620
• Protein ID: WP_000813794.1
• Coordinates: 2427418..2427594 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IF662_RS11615
• Protein ID: WP_001270286.1
• Coordinates: 2426956..2427372 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF662_RS11595	2422273..2423286	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
IF662_RS11600	2423304..2424449	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
IF662_RS11605	2424694..2426100	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
IF662_RS11610	2426184..2426881	-	698	Protein_2269	IS1 family transposase	-
IF662_RS11615	2426956..2427372	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IF662_RS11620	2427418..2427594	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IF662_RS11625	2427816..2428046	+	231	WP_000494244.1	YncJ family protein	-
IF662_RS11630	2428138..2430099	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IF662_RS11635	2430172..2430708	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
IF662_RS11640	2430761..2431975	+	1215	WP_071597387.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2426184..2426561	377
	-	flank	IS/Tn	-	-	2432015..2433280	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T37713 WP_000813794.1 NZ_AP023427:c2427594-2427418 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T37713 NZ_AP023427:c2427594-2427418 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT37713 WP_001270286.1 NZ_AP023427:c2427372-2426956 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT37713 NZ_AP023427:c2427372-2426956 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB