Detailed information of TA system TA037698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2177..2708
• Replicon: plasmid pMM59_02
• Accession: NZ_AP023422
• Organism: Pusillibacter faecalis strain MM59

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KJS55_RS17205
• Protein ID: WP_118626743.1
• Coordinates: 2177..2449 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KJS55_RS17210
• Protein ID: WP_118626744.1
• Coordinates: 2436..2708 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS55_RS17185	1..214	+	214	WP_213543945.1	hypothetical protein	-
KJS55_RS17190 (MM59RIKEN_34750)	477..1436	+	960	WP_118626741.1	hypothetical protein	-
KJS55_RS17195 (MM59RIKEN_34760)	1488..1637	-	150	WP_177610831.1	hypothetical protein	-
KJS55_RS17200 (MM59RIKEN_34770)	1654..2148	-	495	WP_118626742.1	hypothetical protein	-
KJS55_RS17205 (MM59RIKEN_34780)	2177..2449	-	273	WP_118626743.1	type II toxin-antitoxin system YafQ family toxin	Toxin
KJS55_RS17210 (MM59RIKEN_34790)	2436..2708	-	273	WP_118626744.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KJS55_RS17215 (MM59RIKEN_34800)	2942..4444	-	1503	WP_177610833.1	hypothetical protein	-
KJS55_RS17220 (MM59RIKEN_34810)	4830..6134	-	1305	WP_228300620.1	plasmid recombination protein	-
KJS55_RS17225 (MM59RIKEN_34820)	6530..7489	+	960	WP_118626741.1	hypothetical protein	-
KJS55_RS17230 (MM59RIKEN_34830)	7541..7690	-	150	WP_177610831.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-86)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10688.38 Da
• Isoelectric Point: 6.7016

>T37698 WP_118626743.1 NZ_AP023422:c2449-2177 [Pusillibacter faecalis]
MLTIKYQTAFKKDYKRIVRRGYDVRLLEEVISILAEQKPLPEIYRDHNLSGDYIGCRECHITPDWLLIYEIKNDELVLCL
TRTGTHSDLF

Nucleotide

• Length: 273 bp

>T37698 NZ_AP023422:c2449-2177 [Pusillibacter faecalis]
ATGCTGACGATTAAGTATCAGACGGCCTTTAAGAAGGACTATAAGAGAATTGTCCGGAGAGGCTATGACGTGCGGCTGCT
GGAGGAAGTAATTAGTATTTTGGCGGAACAAAAGCCGCTGCCGGAGATATATCGGGATCATAACTTGTCCGGAGATTATA
TCGGGTGCAGGGAATGCCATATAACTCCGGACTGGCTTTTGATCTATGAAATCAAGAATGATGAGCTTGTTCTCTGCTTG
ACCAGAACGGGAACGCATAGTGACTTGTTCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10078.52 Da
• Isoelectric Point: 4.3322

>AT37698 WP_118626744.1 NZ_AP023422:c2708-2436 [Pusillibacter faecalis]
MASTNINIRMDTDLKMQFEAFCADMGMTMTTAFNVFAKKAVREYRIPFEISGEVPNAVTRKAIEDVENGIGMSRAFSSVK
ELMEDLNADD

Nucleotide

• Length: 273 bp

>AT37698 NZ_AP023422:c2708-2436 [Pusillibacter faecalis]
ATGGCAAGCACGAATATCAATATCCGCATGGATACTGACTTGAAGATGCAGTTTGAGGCTTTTTGCGCTGATATGGGAAT
GACGATGACAACCGCTTTCAATGTTTTTGCAAAAAAGGCGGTGCGTGAGTATCGTATTCCCTTTGAGATCAGCGGCGAAG
TGCCGAATGCTGTCACGAGAAAAGCAATCGAGGACGTAGAGAATGGGATTGGAATGAGCAGGGCTTTTTCCTCCGTAAAA
GAGCTGATGGAGGATCTGAATGCTGACGATTAA