Detailed information of TA system TA037655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3294837..3295392
• Replicon: chromosome
• Accession: NZ_AP023354
• Organism: Actinocatenispora sera strain NBRC 101916

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A810L0X2
• Locus tag: Asera_RS15870
• Protein ID: WP_030448747.1
• Coordinates: 3295045..3295392 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Asera_RS15865
• Protein ID: WP_030448746.1
• Coordinates: 3294837..3295058 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Asera_RS15845 (Asera_31670)	3291235..3292047	-	813	WP_051802796.1	thioredoxin domain-containing protein	-
Asera_RS15850 (Asera_31680)	3292500..3292901	+	402	WP_030448743.1	single-stranded DNA-binding protein	-
Asera_RS15855 (Asera_31690)	3292978..3294111	+	1134	WP_051802797.1	glutamate 5-kinase	-
Asera_RS15860 (Asera_31700)	3294132..3294752	+	621	WP_030448745.1	maleylpyruvate isomerase family mycothiol-dependent enzyme	-
Asera_RS15865 (Asera_31710)	3294837..3295058	+	222	WP_030448746.1	ribbon-helix-helix domain-containing protein	Antitoxin
Asera_RS15870 (Asera_31720)	3295045..3295392	+	348	WP_030448747.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Asera_RS15875 (Asera_31730)	3295394..3296200	-	807	WP_051802798.1	helix-turn-helix transcriptional regulator	-
Asera_RS15880 (Asera_31740)	3296256..3297452	+	1197	WP_211255748.1	MFS transporter	-
Asera_RS15885 (Asera_31750)	3297673..3298938	+	1266	WP_030448750.1	glutamate-5-semialdehyde dehydrogenase	-
Asera_RS15890 (Asera_31760)	3299180..3299734	-	555	WP_212804609.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-41)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12537.45 Da
• Isoelectric Point: 10.8352

>T37655 WP_030448747.1 NZ_AP023354:3295045-3295392 [Actinocatenispora sera]
MRRGDVYWVNLDPAWPAECNKIRPAVIVSNDGANRRAEQLGRGVLTVVPVTSNTTRIYPFQVLLPEGAGGLRGAAKAQAE
QVRSVSVGRLTQRLGTLDQPLLADLDRALRLHLAL

Nucleotide

• Length: 348 bp

>T37655 NZ_AP023354:3295045-3295392 [Actinocatenispora sera]
ATGCGGCGCGGTGACGTCTACTGGGTCAATCTCGACCCGGCCTGGCCGGCCGAGTGCAACAAGATCCGTCCGGCCGTGAT
CGTCAGCAACGACGGAGCGAACCGGCGTGCCGAGCAACTGGGCCGCGGCGTGCTCACGGTGGTCCCGGTCACGTCCAACA
CCACCCGGATCTACCCGTTCCAGGTGCTGCTCCCCGAAGGCGCCGGCGGCCTGCGCGGCGCAGCCAAGGCCCAGGCCGAG
CAGGTCCGGTCGGTGTCGGTGGGCCGGCTCACCCAGCGCCTCGGCACCCTGGACCAGCCGCTGCTGGCCGATCTCGACCG
GGCCCTGCGCCTCCACCTCGCCCTCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8216.89 Da
• Isoelectric Point: 4.0987

>AT37655 WP_030448746.1 NZ_AP023354:3294837-3295058 [Actinocatenispora sera]
MKMSVSLPDEDVAFIDEHAARTDAPGRSAVLHEAIRLLRDRELEDQYERAWDEWSTDADGWNATSADGLDAAR

Nucleotide

• Length: 222 bp

>AT37655 NZ_AP023354:3294837-3295058 [Actinocatenispora sera]
ATGAAGATGAGTGTGAGCCTCCCCGACGAGGACGTCGCCTTCATCGACGAGCATGCCGCCCGCACCGATGCGCCCGGCCG
GTCGGCGGTGCTGCACGAGGCCATCCGGCTGTTGCGCGACCGGGAGCTGGAAGACCAGTACGAACGGGCCTGGGACGAGT
GGTCGACCGACGCGGACGGGTGGAACGCGACCAGCGCGGATGGCCTCGATGCGGCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A810L0X2