Detailed information of TA system TA037651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5403279..5403919
• Replicon: chromosome
• Accession: NZ_AP023348
• Organism: Pseudomonas sp. RtIB026

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PRtIB026_RS24350
• Protein ID: WP_203648116.1
• Coordinates: 5403509..5403919 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: PRtIB026_RS24345
• Protein ID: WP_011535889.1
• Coordinates: 5403279..5403509 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PRtIB026_RS24325	5398632..5399957	-	1326	WP_203648112.1	precorrin-3B synthase	-
PRtIB026_RS24330	5400173..5401384	+	1212	WP_203648113.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
PRtIB026_RS24335	5401377..5402471	+	1095	WP_203648114.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
PRtIB026_RS24340	5402468..5403196	+	729	WP_203648115.1	cobalt-precorrin-6A reductase	-
PRtIB026_RS24345	5403279..5403509	+	231	WP_011535889.1	antitoxin	Antitoxin
PRtIB026_RS24350	5403509..5403919	+	411	WP_203648116.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PRtIB026_RS24355	5403992..5404429	+	438	WP_203648117.1	NfeD family protein	-
PRtIB026_RS24360	5404457..5405311	+	855	WP_110735010.1	SPFH/Band 7/PHB domain protein	-
PRtIB026_RS24365	5405545..5405934	+	390	WP_203648118.1	DUF2946 domain-containing protein	-
PRtIB026_RS24370	5405982..5406464	+	483	WP_203648119.1	copper chaperone PCu(A)C	-
PRtIB026_RS24375	5406489..5406920	+	432	WP_203648120.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15246.60 Da
• Isoelectric Point: 9.4581

>T37651 WP_203648116.1 NZ_AP023348:5403509-5403919 [Pseudomonas sp. RtIB026]
MLRYMLDTNICIFTIKNKPQVVREAFNRYHGQLAISTVTLMELIYGAEKSAAPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVSHPR

Nucleotide

• Length: 411 bp

>T37651 NZ_AP023348:5403509-5403919 [Pseudomonas sp. RtIB026]
ATGCTCAGGTACATGCTCGATACCAACATCTGCATTTTCACCATCAAGAACAAGCCACAGGTAGTGCGTGAGGCATTCAA
TCGCTACCACGGCCAGTTGGCTATCAGCACGGTAACCCTGATGGAGTTGATCTACGGTGCGGAGAAATCCGCCGCGCCGG
AACGTAACCTGGCCATTGTCGAAGGGTTCGCCGCACGGCTCGATGTGCTTGACTACGACATTCAGGGCGCAGCTCACACC
GCGCAGCTACGTGCGGAACTGGCCAAGGCCGGCACGCCCATCGGCCCCTATGACAGAATGATTGCCGGCCACGCCCGCGC
TCGTGGCCTCACGCTGGTGACCAACAACCTGCGCGAGTTCCAGCGTGTTCCGGGGTTGCGTGTGGAAGACTGGGTGTCAC
ACCCACGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT37651 WP_011535889.1 NZ_AP023348:5403279-5403509 [Pseudomonas sp. RtIB026]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT37651 NZ_AP023348:5403279-5403509 [Pseudomonas sp. RtIB026]
ATGGAGCAAGGTGCAGTCTTCAAAAGCAACCGCAGCCAGGCTATCCGTATGCCCAAAGCCCTCGCCCTTCCAGAAGACGT
CACCCGCGTGGACATCGTCGCGGTGGGCCGTTCCCGTATCATTTCGCCTGCCGGCGAAAGCTGGGACAGCTGGTTCGAGG
GTGACGATGTGAGTGCGGACTTCATGGCAAGCAGGGAACAGCCAGCCGACCAGGAGCGCGAAGGATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6