Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5171212..5171837 Replicon chromosome
Accession NZ_AP023337
Organism Klebsiella pneumoniae strain MH15-269M

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HRS02_RS25090 Protein ID WP_002882817.1
Coordinates 5171212..5171595 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HRS02_RS25095 Protein ID WP_004150355.1
Coordinates 5171595..5171837 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS02_RS25075 5168578..5169480 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HRS02_RS25080 5169477..5170112 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HRS02_RS25085 5170109..5171038 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HRS02_RS25090 5171212..5171595 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HRS02_RS25095 5171595..5171837 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HRS02_RS25100 5172042..5172959 + 918 WP_021466676.1 alpha/beta hydrolase -
HRS02_RS25105 5172973..5173914 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HRS02_RS25110 5173959..5174396 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HRS02_RS25115 5174393..5175253 - 861 WP_004146232.1 virulence factor BrkB family protein -
HRS02_RS25120 5175247..5175846 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T37635 WP_002882817.1 NZ_AP023337:c5171595-5171212 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T37635 NZ_AP023337:c5171595-5171212 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT37635 WP_004150355.1 NZ_AP023337:c5171837-5171595 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT37635 NZ_AP023337:c5171837-5171595 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References