Detailed information of TA system TA037632

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4598127..4598830
• Replicon: chromosome
• Accession: NZ_AP023337
• Organism: Klebsiella pneumoniae strain MH15-269M

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A3P1KY50
• Locus tag: HRS02_RS22385
• Protein ID: WP_001757895.1
• Coordinates: 4598127..4598468 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A3K4FCA5
• Locus tag: HRS02_RS22390
• Protein ID: WP_024185730.1
• Coordinates: 4598489..4598830 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS02_RS22355	4593140..4593406	+	267	WP_004178415.1	hypothetical protein	-
HRS02_RS22360	4593406..4594078	+	673	Protein_4380	DUF4400 domain-containing protein	-
HRS02_RS22365	4594089..4594973	+	885	WP_004192285.1	RES domain-containing protein	-
HRS02_RS22370	4595172..4595360	-	189	Protein_4382	transposase	-
HRS02_RS22375	4595377..4596488	-	1112	Protein_4383	IS3 family transposase	-
HRS02_RS22380	4596856..4597863	-	1008	WP_001757896.1	restriction endonuclease	-
HRS02_RS22385	4598127..4598468	-	342	WP_001757895.1	TA system toxin CbtA family protein	Toxin
HRS02_RS22390	4598489..4598830	-	342	WP_024185730.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRS02_RS22395	4598841..4599383	-	543	WP_001757893.1	DNA repair protein RadC	-
HRS02_RS22400	4599396..4599836	-	441	WP_001757892.1	antirestriction protein	-
HRS02_RS22405	4599867..4600688	-	822	WP_001757890.1	DUF945 domain-containing protein	-
HRS02_RS22410	4600809..4601282	-	474	WP_024185729.1	hypothetical protein	-
HRS02_RS22415	4601354..4601806	-	453	WP_077264947.1	hypothetical protein	-
HRS02_RS22420	4601842..4602558	-	717	WP_001757887.1	WYL domain-containing protein	-
HRS02_RS22425	4602802..4603677	-	876	WP_001757886.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4596625..4635149	38524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12759.74 Da
• Isoelectric Point: 9.6548

>T37632 WP_001757895.1 NZ_AP023337:c4598468-4598127 [Klebsiella pneumoniae]
MKTLPATNPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQQHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T37632 NZ_AP023337:c4598468-4598127 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGCAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12744.41 Da
• Isoelectric Point: 6.7345

>AT37632 WP_024185730.1 NZ_AP023337:c4598830-4598489 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDMAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT37632 NZ_AP023337:c4598830-4598489 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATATGGCATTCCCGCATTTTATTAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A3P1KY50

Antitoxin

• AlphaFold DB: A0A3K4FCA5