Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1433781..1434517 Replicon chromosome
Accession NZ_AP023337
Organism Klebsiella pneumoniae strain MH15-269M

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag HRS02_RS07040 Protein ID WP_032433360.1
Coordinates 1434035..1434517 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HRS02_RS07035 Protein ID WP_003026799.1
Coordinates 1433781..1434047 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS02_RS07010 1429427..1430566 + 1140 WP_032433368.1 mannitol dehydrogenase -
HRS02_RS07015 1430595..1431257 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
HRS02_RS07020 1431241..1432245 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
HRS02_RS07025 1432263..1432895 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
HRS02_RS07030 1432905..1433468 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
HRS02_RS07035 1433781..1434047 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HRS02_RS07040 1434035..1434517 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
HRS02_RS07045 1434878..1435477 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
HRS02_RS07050 1435690..1436634 - 945 WP_077254249.1 fimbrial protein -
HRS02_RS07055 1436646..1437224 - 579 WP_032432061.1 type 1 fimbrial protein -
HRS02_RS07060 1437228..1437968 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1401726..1445460 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T37620 WP_032433360.1 NZ_AP023337:1434035-1434517 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T37620 NZ_AP023337:1434035-1434517 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT37620 WP_003026799.1 NZ_AP023337:1433781-1434047 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT37620 NZ_AP023337:1433781-1434047 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References