Detailed information of TA system TA037545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4095201..4095777
• Replicon: chromosome
• Accession: NZ_AP023315
• Organism: Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-4614

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: K5684_RS19995
• Protein ID: WP_001131963.1
• Coordinates: 4095490..4095777 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: K5684_RS19990
• Protein ID: WP_000063142.1
• Coordinates: 4095201..4095503 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5684_RS19975 (4091711)	4091711..4093861	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
K5684_RS19980 (4093956)	4093956..4094159	+	204	WP_000460616.1	YbdD/YjiX family protein	-
K5684_RS19985 (4094170)	4094170..4095126	+	957	WP_000187839.1	GTPase	-
K5684_RS19990 (4095201)	4095201..4095503	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
K5684_RS19995 (4095490)	4095490..4095777	-	288	WP_001131963.1	BrnT family toxin	Toxin
K5684_RS20000 (4096046)	4096046..4096960	-	915	WP_000290512.1	restriction endonuclease	-
K5684_RS20005 (4097158)	4097158..4100667	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T37545 WP_001131963.1 NZ_AP023315:c4095777-4095490 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T37545 NZ_AP023315:c4095777-4095490 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT37545 WP_000063142.1 NZ_AP023315:c4095503-4095201 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT37545 NZ_AP023315:c4095503-4095201 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01