Detailed information of TA system TA037447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 123578..124221
• Replicon: plasmid L-4567 strain 4[5]12:i:-
• Accession: NZ_AP023307
• Organism: Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-4567

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: K5646_RS24605
• Protein ID: WP_001044768.1
• Coordinates: 123805..124221 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: K5646_RS24600
• Protein ID: WP_001261287.1
• Coordinates: 123578..123808 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5646_RS24590 (118758)	118758..119891	-	1134	WP_000545983.1	DUF3800 domain-containing protein	-
K5646_RS24920 (120154)	120154..121791	-	1638	WP_223201785.1	hypothetical protein	-
K5646_RS24925 (121842)	121842..122672	-	831	WP_223201786.1	hypothetical protein	-
K5646_RS24930 (122724)	122724..123272	-	549	WP_039002808.1	hypothetical protein	-
K5646_RS24600 (123578)	123578..123808	+	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K5646_RS24605 (123805)	123805..124221	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5646_RS24610 (124383)	124383..126521	-	2139	WP_039002805.1	AAA family ATPase	-
K5646_RS24615 (126986)	126986..127981	+	996	WP_000246636.1	hypothetical protein	-
K5646_RS24620 (128024)	128024..128917	+	894	WP_001553853.1	S-4TM family putative pore-forming effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / sul3 / blaTEM-1B / aac(3)-IId / mcr-3.1	-	1..131843	131843
	-	flank	IS/Tn	-	-	129054..129557	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T37447 WP_001044768.1 NZ_AP023307:123805-124221 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T37447 NZ_AP023307:123805-124221 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT37447 WP_001261287.1 NZ_AP023307:123578-123808 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT37447 NZ_AP023307:123578-123808 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4