Detailed information of TA system TA037434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4095248..4095824
• Replicon: chromosome
• Accession: NZ_AP023306
• Organism: Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-4567

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: K5646_RS19985
• Protein ID: WP_001131963.1
• Coordinates: 4095537..4095824 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: K5646_RS19980
• Protein ID: WP_000063142.1
• Coordinates: 4095248..4095550 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5646_RS19965 (4091758)	4091758..4093908	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
K5646_RS19970 (4094003)	4094003..4094206	+	204	WP_000460616.1	YbdD/YjiX family protein	-
K5646_RS19975 (4094217)	4094217..4095173	+	957	WP_000187839.1	GTPase	-
K5646_RS19980 (4095248)	4095248..4095550	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
K5646_RS19985 (4095537)	4095537..4095824	-	288	WP_001131963.1	BrnT family toxin	Toxin
K5646_RS19990 (4096093)	4096093..4097007	-	915	WP_000290512.1	restriction endonuclease	-
K5646_RS19995 (4097205)	4097205..4100714	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T37434 WP_001131963.1 NZ_AP023306:c4095824-4095537 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T37434 NZ_AP023306:c4095824-4095537 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT37434 WP_000063142.1 NZ_AP023306:c4095550-4095248 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT37434 NZ_AP023306:c4095550-4095248 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01