Detailed information of TA system TA037362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2480..3015
• Replicon: plasmid L-4445 strain 4[5]12:i:-
• Accession: NZ_AP023301
• Organism: Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-4445

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: K5630_RS23900
• Protein ID: WP_000222766.1
• Coordinates: 2728..3015 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: K5630_RS23895
• Protein ID: WP_001132900.1
• Coordinates: 2480..2731 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5630_RS23880 (101)	101..358	+	258	WP_001541895.1	replication regulatory protein RepA	-
K5630_RS23885 (590)	590..664	+	75	WP_061357229.1	RepA leader peptide Tap	-
K5630_RS23890 (657)	657..1514	+	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
K5630_RS23895 (2480)	2480..2731	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K5630_RS23900 (2728)	2728..3015	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5630_RS23905 (3090)	3090..3278	-	189	Protein_5	IS3 family transposase	-
K5630_RS23910 (3312)	3312..3812	-	501	Protein_6	Tn3 family transposase	-
K5630_RS23915 (3846)	3846..4892	-	1047	Protein_7	Tn3 family transposase	-
K5630_RS24885 (4926)	4926..5006	-	81	Protein_8	MerR family transcriptional regulator	-
K5630_RS23920 (5067)	5067..5771	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
K5630_RS23925 (5965)	5965..6351	+	387	WP_063102497.1	bleomycin binding protein	-
K5630_RS23930 (6671)	6671..6892	-	222	WP_226909166.1	hypothetical protein	-
K5630_RS23935 (6876)	6876..7697	-	822	WP_039026397.1	IS3 family transposase	-
K5630_RS23940 (7694)	7694..8008	-	315	WP_039026396.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-3.1 / aac(3)-IId / blaTEM-1B / sul3 / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M)	-	1..131843	131843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T37362 WP_000222766.1 NZ_AP023301:2728-3015 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T37362 NZ_AP023301:2728-3015 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT37362 WP_001132900.1 NZ_AP023301:2480-2731 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT37362 NZ_AP023301:2480-2731 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2