Detailed information of TA system TA037260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4148055..4148631
• Replicon: chromosome
• Accession: NZ_AP023291
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain L-4126

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: K5613_RS20330
• Protein ID: WP_001131963.1
• Coordinates: 4148344..4148631 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: K5613_RS20325
• Protein ID: WP_000063142.1
• Coordinates: 4148055..4148357 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5613_RS20305 (4143794)	4143794..4144198	-	405	Protein_3987	Tar ligand binding domain-containing protein	-
K5613_RS20310 (4144565)	4144565..4146715	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
K5613_RS20315 (4146810)	4146810..4147013	+	204	WP_000460616.1	YbdD/YjiX family protein	-
K5613_RS20320 (4147024)	4147024..4147980	+	957	WP_000187839.1	GTPase	-
K5613_RS20325 (4148055)	4148055..4148357	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
K5613_RS20330 (4148344)	4148344..4148631	-	288	WP_001131963.1	BrnT family toxin	Toxin
K5613_RS20335 (4148900)	4148900..4149814	-	915	WP_000290512.1	restriction endonuclease	-
K5613_RS20340 (4150012)	4150012..4153521	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T37260 WP_001131963.1 NZ_AP023291:c4148631-4148344 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T37260 NZ_AP023291:c4148631-4148344 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT37260 WP_000063142.1 NZ_AP023291:c4148357-4148055 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT37260 NZ_AP023291:c4148357-4148055 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01