Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 224153..224913 Replicon chromosome
Accession NZ_AP023288
Organism Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-3835

Toxin (Protein)


Gene name tacT Uniprot ID Q57IH1
Locus tag K5610_RS01090 Protein ID WP_000533909.1
Coordinates 224428..224913 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID M7RHS4
Locus tag K5610_RS01085 Protein ID WP_000965886.1
Coordinates 224153..224440 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5610_RS01060 (219448) 219448..219750 + 303 WP_000605590.1 YsaB family lipoprotein -
K5610_RS01065 (219889) 219889..220800 + 912 WP_001168551.1 glycine--tRNA ligase subunit alpha -
K5610_RS01070 (220810) 220810..222879 + 2070 WP_001291736.1 glycine--tRNA ligase subunit beta -
K5610_RS01075 (223061) 223061..223336 + 276 Protein_212 IS3 family transposase -
K5610_RS01080 (223508) 223508..223975 + 468 WP_000702452.1 GNAT family N-acetyltransferase -
K5610_RS01085 (224153) 224153..224440 + 288 WP_000965886.1 DUF1778 domain-containing protein Antitoxin
K5610_RS01090 (224428) 224428..224913 + 486 WP_000533909.1 GNAT family N-acetyltransferase Toxin
K5610_RS01095 (225285) 225285..225824 - 540 WP_000047149.1 copper-binding periplasmic metallochaperone CueP -
K5610_RS01100 (225998) 225998..226210 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
K5610_RS01105 (226499) 226499..226789 - 291 WP_000455790.1 HTH-type transcriptional regulator -
K5610_RS01110 (227228) 227228..227938 + 711 WP_000190524.1 DUF3053 domain-containing protein -
K5610_RS01115 (227988) 227988..228962 - 975 WP_000804674.1 glyoxylate/hydroxypyruvate reductase GhrB -
K5610_RS01120 (229181) 229181..229843 - 663 WP_000747548.1 OmpA family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(31-152)

Antitoxin

Vibrio phage ICP1, Orf50

(5-94)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17703.41 Da        Isoelectric Point: 9.8719

>T37169 WP_000533909.1 NZ_AP023288:224428-224913 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKSSQTQQRTLFLRLP
Q

Download         Length: 486 bp

>T37169 NZ_AP023288:224428-224913 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10950.60 Da        Isoelectric Point: 4.6811

>AT37169 WP_000965886.1 NZ_AP023288:224153-224440 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILETACQAAEKVILDRRVFNFNDEQYEEFINLLDAPVADD
PVIEKLLARKPQWDV

Download         Length: 288 bp

>AT37169 NZ_AP023288:224153-224440 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7F36
PDB 7AK8
PDB 5FVJ

Antitoxin

Source ID Structure
PDB 7AK8
AlphaFold DB A0A3V2JDX2

References