Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4018836..4019412 Replicon chromosome
Accession NZ_AP023286
Organism Escherichia coli strain 2018-18.25PC

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JMH23_RS19255 Protein ID WP_001297643.1
Coordinates 4019125..4019412 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag JMH23_RS19250 Protein ID WP_000063156.1
Coordinates 4018836..4019138 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMH23_RS19230 4014527..4015039 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
JMH23_RS19235 4015432..4017582 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
JMH23_RS19240 4017632..4017835 + 204 WP_000467859.1 YbdD/YjiX family protein -
JMH23_RS19245 4017846..4018802 + 957 WP_032292598.1 GTPase -
JMH23_RS19250 4018836..4019138 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
JMH23_RS19255 4019125..4019412 - 288 WP_001297643.1 BrnT family toxin Toxin
JMH23_RS19260 4019611..4020307 - 697 Protein_3764 IS1 family transposase -
JMH23_RS19265 4020633..4021865 + 1233 WP_032292597.1 multidrug efflux MFS transporter MdtM -
JMH23_RS19270 4021906..4023186 + 1281 WP_016230805.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4019611..4019820 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T37162 WP_001297643.1 NZ_AP023286:c4019412-4019125 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T37162 NZ_AP023286:c4019412-4019125 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT37162 WP_000063156.1 NZ_AP023286:c4019138-4018836 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT37162 NZ_AP023286:c4019138-4018836 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References