Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 849899..850553 Replicon chromosome
Accession NZ_AP023286
Organism Escherichia coli strain 2018-18.25PC

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JMH23_RS04095 Protein ID WP_000244777.1
Coordinates 850146..850553 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JMH23_RS04090 Protein ID WP_000354046.1
Coordinates 849899..850165 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMH23_RS04065 845068..845811 + 744 WP_000951964.1 SDR family oxidoreductase -
JMH23_RS04070 845868..847301 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JMH23_RS04075 847346..847657 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JMH23_RS04080 847821..848480 + 660 WP_000250274.1 hemolysin III family protein -
JMH23_RS04085 848676..849656 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JMH23_RS04090 849899..850165 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JMH23_RS04095 850146..850553 + 408 WP_000244777.1 toxin CptA Toxin
JMH23_RS04100 850593..851114 - 522 WP_001055874.1 flavodoxin FldB -
JMH23_RS04105 851226..852122 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JMH23_RS04110 852147..852857 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JMH23_RS04115 852863..854596 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T37146 WP_000244777.1 NZ_AP023286:850146-850553 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T37146 NZ_AP023286:850146-850553 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT37146 WP_000354046.1 NZ_AP023286:849899-850165 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT37146 NZ_AP023286:849899-850165 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References