Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 112190..113676 Replicon plasmid pRvVAR031b
Accession NZ_AP023271
Organism Agrobacterium vitis strain VAR03-1

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A1S2DSH6
Locus tag RvVAT039_RS24600 Protein ID WP_070149873.1
Coordinates 112190..113452 (-) Length 421 a.a.

Antitoxin (Protein)


Gene name HipB2 Uniprot ID A0A1S2DU60
Locus tag RvVAT039_RS24605 Protein ID WP_070149959.1
Coordinates 113449..113676 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RvVAT039_RS24580 107323..108591 - 1269 WP_071201628.1 glycerate kinase -
RvVAT039_RS24585 108588..109682 - 1095 WP_071207341.1 tartrate dehydrogenase -
RvVAT039_RS24590 109704..111005 - 1302 WP_046802348.1 MFS transporter -
RvVAT039_RS24595 111129..112043 + 915 WP_071201483.1 LysR family transcriptional regulator -
RvVAT039_RS24600 112190..113452 - 1263 WP_070149873.1 type II toxin-antitoxin system HipA family toxin Toxin
RvVAT039_RS24605 113449..113676 - 228 WP_070149959.1 helix-turn-helix transcriptional regulator Antitoxin
RvVAT039_RS24610 113824..114735 - 912 WP_070149872.1 HNH endonuclease -
RvVAT039_RS24615 114891..117239 + 2349 WP_071201484.1 hypothetical protein -
RvVAT039_RS24620 117494..118132 - 639 WP_070149870.1 ATP-binding cassette domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..161351 161351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(147-377)

Antitoxin

Cro/C1-type helix-turn-helix domain

(11-63)


Sequences

Toxin        


Download         Length: 421 a.a.        Molecular weight: 45829.64 Da        Isoelectric Point: 5.5201

>T37142 WP_070149873.1 NZ_AP023271:c113452-112190 [Agrobacterium vitis]
MTRVLDVYFRDTKAGVLGRLDDGLLTFAYDGAYLLDQRSRAISFSMPLQEEPFGDQVVRPFFSGLLPDEGARQRLAGALG
LSAGNAFGLLEVIGGECAGALSLYPTGTAPEPPGQSQAEILTSERLDEILSRLRTRPLLGGDEGIRLSLAGAQDKLAVIV
DGDSIALARDGRPTTHILKPVIQALEGTVENEYFCMRLAARLKLPVPHVEIRLDGTTAFLLVERYDRMKAADGIIERLHQ
EDLCQALSVPPELKYEAEGGPGTERALGLIDQACARPVADRLQFIRMLIFHYLVGNADAHGKNYALLYGSNEPDLAPTYD
VVCTAVYPRLSKRLAMKIGGRDVPDTIQLKHWKALVPDTKSSQRILVRDLAQMAERIVSEADTLVAELADEGIRHPILSA
IRKVISSRAGLLQRAAEQVS

Download         Length: 1263 bp

>T37142 NZ_AP023271:c113452-112190 [Agrobacterium vitis]
ATGACCCGGGTATTGGACGTCTATTTTCGCGACACCAAGGCGGGCGTACTCGGCCGGCTTGACGACGGCCTGCTGACTTT
TGCCTACGATGGCGCGTATCTCCTGGACCAACGATCGCGTGCGATCTCGTTCTCGATGCCCCTGCAAGAAGAACCCTTTG
GTGATCAGGTGGTGCGCCCGTTTTTCTCCGGTCTCTTGCCGGACGAAGGCGCGCGGCAACGCCTAGCTGGCGCGCTCGGC
CTTTCGGCAGGCAACGCCTTCGGGCTACTGGAAGTTATCGGGGGCGAATGCGCAGGTGCCTTGTCACTCTATCCCACCGG
CACGGCGCCCGAACCTCCCGGCCAGTCCCAAGCCGAAATTCTCACTTCGGAACGTCTGGATGAAATTCTCAGCAGGCTGC
GGACCCGGCCTTTGCTTGGCGGAGACGAGGGCATTCGCCTATCGCTCGCCGGCGCGCAGGATAAACTGGCGGTCATTGTC
GATGGAGATTCCATTGCCTTGGCGAGAGATGGTCGACCAACGACCCATATCTTGAAACCAGTCATCCAGGCGTTGGAAGG
CACGGTGGAGAACGAGTATTTTTGTATGCGGCTGGCTGCCCGCCTCAAGCTGCCGGTACCCCATGTCGAGATACGCCTAG
ATGGGACGACTGCCTTTCTCTTGGTTGAACGCTATGATCGGATGAAGGCTGCAGACGGCATCATTGAGCGCCTACACCAG
GAAGACCTTTGCCAAGCCCTTAGCGTACCACCGGAGCTGAAGTATGAGGCCGAGGGCGGACCGGGTACAGAACGGGCTCT
CGGGCTGATCGACCAGGCCTGCGCTAGACCTGTCGCGGATCGCTTACAGTTCATCCGCATGCTGATCTTCCACTATCTCG
TCGGCAACGCCGATGCCCACGGCAAGAATTACGCCCTGCTTTATGGCTCCAATGAACCGGATCTGGCACCGACCTACGAT
GTCGTTTGCACGGCTGTTTATCCCCGCTTGTCAAAGAGGCTCGCCATGAAGATTGGCGGCCGCGATGTGCCCGACACCAT
TCAGCTGAAGCATTGGAAGGCATTGGTGCCCGACACTAAATCTTCCCAGCGCATCCTTGTTCGCGATCTTGCCCAGATGG
CGGAAAGGATTGTGAGCGAGGCCGATACCCTTGTTGCAGAACTGGCCGACGAGGGAATCAGGCACCCCATCCTGAGTGCA
ATTCGCAAGGTCATCTCAAGTCGGGCTGGTTTGCTACAGAGAGCCGCGGAGCAGGTGTCTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8219.65 Da        Isoelectric Point: 11.4487

>AT37142 WP_070149959.1 NZ_AP023271:c113676-113449 [Agrobacterium vitis]
MEKIKDTRSLGMLIRQERKQQGLTQEQLAGVIGVGVRFLRELEAGKESCQIGRAMQVLAGLGLVVSVGRRQGPRS

Download         Length: 228 bp

>AT37142 NZ_AP023271:c113676-113449 [Agrobacterium vitis]
ATGGAAAAGATCAAAGATACGAGAAGCCTCGGCATGCTGATCCGTCAGGAGCGCAAACAGCAAGGGCTAACCCAGGAGCA
ACTCGCAGGGGTGATTGGTGTGGGCGTACGTTTTCTGCGTGAACTCGAGGCCGGCAAAGAGAGCTGCCAGATTGGCCGTG
CGATGCAAGTCCTGGCTGGTTTGGGCCTCGTTGTTTCGGTCGGGCGTCGCCAGGGCCCGCGATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2DSH6

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2DU60

References