Detailed information of TA system TA037106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 152170..152982
• Replicon: plasmid MesoLjLa2
• Accession: NZ_AP023258
• Organism: Mesorhizobium sp. L-2-11

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JG739_RS32250
• Protein ID: WP_202367828.1
• Coordinates: 152473..152982 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: JG739_RS32245
• Protein ID: WP_202367827.1
• Coordinates: 152170..152469 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG739_RS32225	147959..148807	-	849	WP_202367823.1	ABC transporter permease	-
JG739_RS32230	148833..150197	-	1365	WP_202367824.1	LLM class flavin-dependent oxidoreductase	-
JG739_RS32235	150284..150841	-	558	WP_202367825.1	FMN reductase	-
JG739_RS32240	151188..151985	-	798	WP_202367826.1	hypothetical protein	-
JG739_RS32245	152170..152469	+	300	WP_202367827.1	DUF1778 domain-containing protein	Antitoxin
JG739_RS32250	152473..152982	+	510	WP_202367828.1	GNAT family N-acetyltransferase	Toxin
JG739_RS32255	152992..153294	+	303	WP_202367829.1	hypothetical protein	-
JG739_RS32260	153692..153910	-	219	Protein_144	formyltetrahydrofolate deformylase	-
JG739_RS32265	154232..157207	-	2976	WP_202367830.1	Tn3 family transposase	-
JG739_RS32270	157362..157955	+	594	WP_202367916.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..287750	287750

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 17791.69 Da
• Isoelectric Point: 7.8067

>T37106 WP_202367828.1 NZ_AP023258:152473-152982 [Mesorhizobium sp. L-2-11]
MALSPPEPLTAAHDVSEFTCGKPTLDHWLMTRALSNQEKGFTAVLVVHEAGRVVGYYGLAPTAVVPGVLPRSIRTGQPPD
PVPCLLLGQLATDIGWAGRGIGTGLLKHALERCVTAAALVGGRALMVNAVDDEAAEFWRRRGFLPSKDDPLVLFRSMAAI
IASIAAGKG

Nucleotide

• Length: 510 bp

>T37106 NZ_AP023258:152473-152982 [Mesorhizobium sp. L-2-11]
TTGGCCCTCTCGCCTCCCGAACCGCTCACCGCGGCTCACGACGTTTCCGAGTTCACCTGCGGAAAACCGACTCTCGACCA
TTGGCTCATGACCCGTGCGCTCTCCAACCAGGAGAAGGGCTTCACCGCCGTCCTGGTCGTGCATGAGGCGGGACGTGTGG
TCGGCTACTATGGCCTCGCGCCGACCGCAGTCGTTCCGGGCGTCCTGCCGCGCTCCATCCGCACCGGCCAACCGCCCGAT
CCAGTGCCCTGCCTTCTGCTCGGGCAACTTGCGACCGACATCGGCTGGGCAGGGCGCGGGATCGGCACAGGCCTGCTCAA
GCACGCTCTTGAGCGCTGCGTGACCGCGGCTGCGCTCGTCGGCGGTCGCGCGCTGATGGTCAACGCCGTCGACGACGAGG
CTGCAGAATTCTGGCGGCGGCGCGGATTCCTTCCATCGAAGGACGACCCCCTGGTCCTGTTCCGCTCAATGGCAGCGATC
ATCGCCTCAATCGCCGCAGGAAAGGGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10893.55 Da
• Isoelectric Point: 5.8835

>AT37106 WP_202367827.1 NZ_AP023258:152170-152469 [Mesorhizobium sp. L-2-11]
MTTSAERKEHPISMRLPEADVAMIDRAAGLRGRSRTDFVREAAVRAAEDVLMENRLIRMSPEGFADFMAVLSGPATPVPE
IVELAKRPAPWEPGYVAKS

Nucleotide

• Length: 300 bp

>AT37106 NZ_AP023258:152170-152469 [Mesorhizobium sp. L-2-11]
ATGACCACCAGCGCTGAACGCAAGGAACACCCGATCTCGATGCGCCTCCCCGAGGCCGACGTCGCAATGATCGATCGCGC
CGCCGGCCTGCGCGGCCGATCACGCACGGACTTCGTGCGCGAGGCTGCCGTTCGCGCCGCCGAGGACGTATTGATGGAAA
ACCGGCTCATTCGCATGAGCCCAGAGGGCTTCGCCGACTTCATGGCGGTCCTGTCCGGACCTGCGACCCCGGTGCCCGAG
ATCGTTGAACTGGCAAAGCGTCCGGCGCCGTGGGAACCCGGCTACGTCGCAAAAAGCTGA