Detailed information of TA system TA037085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 256655..257256
• Replicon: plasmid MesoLj113b2
• Accession: NZ_AP023244
• Organism: Mesorhizobium sp. 113-3-3

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: JG746_RS35350
• Protein ID: WP_199202143.1
• Coordinates: 256655..256978 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: JG746_RS35355
• Protein ID: WP_123170493.1
• Coordinates: 256975..257256 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG746_RS35335	251715..253073	+	1359	WP_199202140.1	type IV secretion system protein VirB10	-
JG746_RS35340	253039..254085	+	1047	WP_199202141.1	P-type DNA transfer ATPase VirB11	-
JG746_RS35345	254063..256396	+	2334	WP_199202142.1	type IV secretory system conjugative DNA transfer family protein	-
JG746_RS35350	256655..256978	+	324	WP_199202143.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JG746_RS35355	256975..257256	+	282	WP_123170493.1	helix-turn-helix domain-containing protein	Antitoxin
JG746_RS35360	257391..259658	-	2268	WP_199202144.1	relaxase/mobilization nuclease domain-containing protein	-
JG746_RS35365	259655..259972	-	318	WP_199202354.1	hypothetical protein	-
JG746_RS35370	260393..261085	+	693	WP_199202145.1	ParA family protein	-
JG746_RS35375	261082..261423	+	342	WP_199202146.1	hypothetical protein	-
JG746_RS35380	261637..261936	+	300	WP_199202147.1	WGR domain-containing protein	-
JG746_RS35385	261949..262194	+	246	WP_199202148.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..353699	353699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-101)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-90)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12329.19 Da
• Isoelectric Point: 10.1923

>T37085 WP_199202143.1 NZ_AP023244:256655-256978 [Mesorhizobium sp. 113-3-3]
MKVIEWLGSSRADVRAFPDDARIEAGWQLELVQRGDDPDDWKPMQIVGAGVREIRVREASGAFRVIYLATLEDRVLVLHA
FQKKIQATSRKDLDLAAQRLKRWKAER

Nucleotide

• Length: 324 bp

>T37085 NZ_AP023244:256655-256978 [Mesorhizobium sp. 113-3-3]
ATGAAGGTGATTGAATGGCTTGGAAGCAGCAGAGCGGATGTTCGAGCGTTTCCTGACGATGCACGGATAGAGGCCGGCTG
GCAGCTCGAACTTGTCCAGCGCGGCGACGATCCCGACGACTGGAAACCGATGCAGATCGTAGGGGCCGGCGTCAGGGAAA
TCAGGGTTCGGGAGGCATCGGGAGCGTTTCGCGTCATCTATCTGGCAACACTAGAGGACCGCGTTTTGGTGCTTCATGCG
TTCCAGAAGAAGATACAGGCCACATCCAGGAAAGACCTCGATCTAGCGGCTCAACGGCTAAAACGATGGAAGGCAGAACG
ATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10501.01 Da
• Isoelectric Point: 5.8769

>AT37085 WP_123170493.1 NZ_AP023244:256975-257256 [Mesorhizobium sp. 113-3-3]
MNVEIYENVWDALADTEQEAANLKARSALLYEIRKAVQRWDIPQEEAAKRLGLTRPRTNDLLRGKLAKFSLDALVNIAAS
AHLDIEIRVKEAA

Nucleotide

• Length: 282 bp

>AT37085 NZ_AP023244:256975-257256 [Mesorhizobium sp. 113-3-3]
ATGAACGTAGAAATCTATGAGAACGTCTGGGATGCCCTGGCGGACACCGAACAGGAGGCAGCAAACCTTAAAGCGCGCTC
GGCGCTGCTCTACGAAATCCGTAAGGCAGTCCAGCGTTGGGACATTCCTCAGGAGGAAGCCGCCAAGCGGCTCGGCCTGA
CGCGTCCTCGCACCAATGATCTGCTGCGCGGTAAGCTCGCCAAATTCTCGCTTGACGCACTTGTCAACATCGCCGCCTCC
GCACACCTGGATATAGAAATCCGGGTAAAAGAGGCTGCATAA