Detailed information of TA system TA037072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1346688..1347293
• Replicon: chromosome
• Accession: NZ_AP023239
• Organism: Paenibacillus sp. URB8-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: X4ZF21
• Locus tag: PUR_RS06280
• Protein ID: WP_025333625.1
• Coordinates: 1346943..1347293 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PUR_RS06275
• Protein ID: WP_175491834.1
• Coordinates: 1346688..1346939 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PUR_RS06260	1342532..1343416	+	885	WP_179034502.1	Abi family protein	-
PUR_RS06265	1343985..1345070	+	1086	WP_179034503.1	outer membrane lipoprotein-sorting protein	-
PUR_RS06270	1345265..1346467	+	1203	WP_179034504.1	alanine racemase	-
PUR_RS06275	1346688..1346939	+	252	WP_175491834.1	ribbon-helix-helix protein, CopG family	Antitoxin
PUR_RS06280	1346943..1347293	+	351	WP_025333625.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PUR_RS06285	1347516..1349705	+	2190	WP_179037776.1	RNA-binding transcriptional accessory protein	-
PUR_RS06290	1349862..1350839	-	978	WP_179034505.1	S-layer homology domain-containing protein	-
PUR_RS06295	1351170..1351640	+	471	WP_179034506.1	ribose 5-phosphate isomerase B	-
PUR_RS06300	1351805..1352110	-	306	WP_179034507.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12794.71 Da
• Isoelectric Point: 5.1284

>T37072 WP_025333625.1 NZ_AP023239:1346943-1347293 [Paenibacillus sp. URB8-2]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIEAASHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T37072 NZ_AP023239:1346943-1347293 [Paenibacillus sp. URB8-2]
TTGATCGTAAAACGCGGCGACGTTTTTTTTGCCGACCTTTCGCCAGTAGTGGGTTCGGAGCAGGGCGGAGTAAGGCCCGT
TCTTGTCATTCAGAACGATATTGGCAATCGCTTCAGTCCTACGGTAATTGTCGCGGCAATTACCGCACAAATTCAAAAGG
CCAAGCTGCCTACTCATGTAGAGATTGAAGCGGCGTCCCATGGCTTTGACCGGGATTCCGTCATTCTGCTGGAGCAGGTT
CGGACGATCGACAAGCAGCGCTTAACGGATAAGATCACCCACCTGGATGACGAAACGATGAAAAAGGTGGATGACTCGCT
GCAGATCAGCTTGGGTCTGATTGATTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9505.98 Da
• Isoelectric Point: 5.1184

>AT37072 WP_175491834.1 NZ_AP023239:1346688-1346939 [Paenibacillus sp. URB8-2]
MISLPDHLLQEVDGIVQMENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT37072 NZ_AP023239:1346688-1346939 [Paenibacillus sp. URB8-2]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAGGTGGACGGAATTGTCCAGATGGAGAATTCCAACCGCAGTGAATTGAT
CCGGCAGGCCATGAAGCTGTATCTCAGCGAACGGAGGAAGCGTTCCATACGGGAGTCCATGCAGCGTGGATACATGGAAA
TGGCCAAGATCAACTTGACCATGGCATGCGAAGCGTTCTTAGCCGAGGAAGATGCAGACAGCACTCTTGGCCGCTTAGTA
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: X4ZF21