Detailed information of TA system TA037052

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1871646..1872379
• Replicon: chromosome
• Accession: NZ_AP023237
• Organism: Escherichia coli strain F070

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FJI53_RS08945
• Protein ID: WP_176486840.1
• Coordinates: 1871646..1871921 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: FJI53_RS08950
• Protein ID: WP_001360327.1
• Coordinates: 1872011..1872379 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJI53_RS08910	1866808..1866990	-	183	WP_032181276.1	ethanolamine utilization protein	-
FJI53_RS08915	1867444..1868472	-	1029	Protein_1745	IS110 family transposase	-
FJI53_RS08920	1868631..1868744	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FJI53_RS08925	1868757..1868963	-	207	WP_000976829.1	hypothetical protein	-
FJI53_RS08930	1868960..1869079	-	120	Protein_1748	type IV toxin-antitoxin system toxin CbtA	-
FJI53_RS08935	1869286..1870827	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
FJI53_RS08940	1870842..1871588	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
FJI53_RS08945	1871646..1871921	-	276	WP_176486840.1	TA system toxin CbtA family protein	Toxin
FJI53_RS08950	1872011..1872379	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FJI53_RS08955	1872542..1872763	-	222	WP_000692345.1	DUF987 domain-containing protein	-
FJI53_RS08960	1872826..1873302	-	477	WP_001186747.1	RadC family protein	-
FJI53_RS08965	1873318..1873791	-	474	WP_000855059.1	antirestriction protein	-
FJI53_RS08970	1874133..1874951	-	819	WP_088540043.1	DUF945 domain-containing protein	-
FJI53_RS08975	1875106..1875264	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-82)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10315.66 Da
• Isoelectric Point: 4.7439

>T37052 WP_176486840.1 NZ_AP023237:c1871921-1871646 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGAYYREGDR

Nucleotide

• Length: 276 bp

>T37052 NZ_AP023237:c1871921-1871646 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGTGCGTATTACCGTGAAGGAGATCGGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT37052 WP_001360327.1 NZ_AP023237:c1872379-1872011 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT37052 NZ_AP023237:c1872379-1872011 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9IW59