Detailed information of TA system TA037017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 82593..83187
• Replicon: plasmid pYJ4-a
• Accession: NZ_AP023232
• Organism: Escherichia coli strain YJ4

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HXS62_RS23995
• Protein ID: WP_001403920.1
• Coordinates: 82593..82868 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: HXS62_RS24000
• Protein ID: WP_001261274.1
• Coordinates: 82957..83187 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS62_RS23955	77712..77980	+	269	Protein_88	type II toxin-antitoxin system ParD family antitoxin	-
HXS62_RS23960	78096..78257	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HXS62_RS23965	78324..78506	+	183	WP_042004909.1	hypothetical protein	-
HXS62_RS23970	78627..79367	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
HXS62_RS23975	79690..80679	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
HXS62_RS23980	81567..81836	+	270	WP_176486944.1	DUF1778 domain-containing protein	-
HXS62_RS23985	81840..82370	+	531	WP_000348883.1	GNAT family N-acetyltransferase	-
HXS62_RS23990	82371..82588	+	218	Protein_95	transposase	-
HXS62_RS23995	82593..82868	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
HXS62_RS24000	82957..83187	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HXS62_RS24005	83386..86007	-	2622	WP_001586230.1	hypothetical protein	-
HXS62_RS24010	86398..86883	+	486	WP_001403958.1	hypothetical protein	-
HXS62_RS24015	86912..87684	+	773	Protein_100	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaCTX-M-15 / blaTEM-1B / tet(B)	-	1..130952	130952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T37017 WP_001403920.1 NZ_AP023232:c82868-82593 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T37017 NZ_AP023232:c82868-82593 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT37017 WP_001261274.1 NZ_AP023232:c83187-82957 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT37017 NZ_AP023232:c83187-82957 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1