Detailed information of TA system TA037016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 81567..82370
• Replicon: plasmid pYJ4-a
• Accession: NZ_AP023232
• Organism: Escherichia coli strain YJ4

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: HXS62_RS23985
• Protein ID: WP_000348883.1
• Coordinates: 81840..82370 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: HXS62_RS23980
• Protein ID: WP_176486944.1
• Coordinates: 81567..81836 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS62_RS23950	76676..77416	+	741	WP_029702141.1	tyrosine-type recombinase/integrase	-
HXS62_RS23955	77712..77980	+	269	Protein_88	type II toxin-antitoxin system ParD family antitoxin	-
HXS62_RS23960	78096..78257	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HXS62_RS23965	78324..78506	+	183	WP_042004909.1	hypothetical protein	-
HXS62_RS23970	78627..79367	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
HXS62_RS23975	79690..80679	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
HXS62_RS23980	81567..81836	+	270	WP_176486944.1	DUF1778 domain-containing protein	Antitoxin
HXS62_RS23985	81840..82370	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
HXS62_RS23990	82371..82588	+	218	Protein_95	transposase	-
HXS62_RS23995	82593..82868	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
HXS62_RS24000	82957..83187	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HXS62_RS24005	83386..86007	-	2622	WP_001586230.1	hypothetical protein	-
HXS62_RS24010	86398..86883	+	486	WP_001403958.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaCTX-M-15 / blaTEM-1B / tet(B)	-	1..130952	130952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T37016 WP_000348883.1 NZ_AP023232:81840-82370 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T37016 NZ_AP023232:81840-82370 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.62 Da
• Isoelectric Point: 6.4702

>AT37016 WP_176486944.1 NZ_AP023232:81567-81836 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNTPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT37016 NZ_AP023232:81567-81836 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATACGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8