Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1508795..1509371 Replicon chromosome
Accession NZ_AP023230
Organism Escherichia coli strain YJ4

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HXS62_RS07250 Protein ID WP_001297643.1
Coordinates 1509084..1509371 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HXS62_RS07245 Protein ID WP_000063156.1
Coordinates 1508795..1509097 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS62_RS07230 1505391..1507541 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
HXS62_RS07235 1507591..1507794 + 204 WP_000467859.1 YbdD/YjiX family protein -
HXS62_RS07240 1507805..1508761 + 957 WP_001297640.1 GTPase -
HXS62_RS07245 1508795..1509097 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HXS62_RS07250 1509084..1509371 - 288 WP_001297643.1 BrnT family toxin Toxin
HXS62_RS07255 1509570..1510267 - 698 Protein_1408 IS1 family transposase -
HXS62_RS07260 1510324..1510419 + 96 Protein_1409 dienelactone hydrolase family protein -
HXS62_RS07265 1510565..1511797 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HXS62_RS07270 1511838..1513118 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 1438957..1510073 71116


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T36992 WP_001297643.1 NZ_AP023230:c1509371-1509084 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T36992 NZ_AP023230:c1509371-1509084 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT36992 WP_000063156.1 NZ_AP023230:c1509097-1508795 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT36992 NZ_AP023230:c1509097-1508795 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References