Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 22691..23283 Replicon plasmid pYJ3-b
Accession NZ_AP023229
Organism Escherichia coli strain YJ3

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag HXS63_RS24505 Protein ID WP_000520549.1
Coordinates 22691..22939 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag HXS63_RS24510 Protein ID WP_000681613.1
Coordinates 22936..23283 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS63_RS24475 19575..20090 + 516 WP_001025395.1 J domain-containing protein -
HXS63_RS24480 20195..20737 + 543 WP_001420167.1 hypothetical protein -
HXS63_RS24485 20734..20985 + 252 WP_086621303.1 hypothetical protein -
HXS63_RS24490 20972..21199 + 228 WP_000801440.1 hypothetical protein -
HXS63_RS24495 21642..21941 + 300 WP_142284800.1 hypothetical protein -
HXS63_RS24500 22036..22386 + 351 WP_063848416.1 hypothetical protein -
HXS63_RS24505 22691..22939 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
HXS63_RS24510 22936..23283 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HXS63_RS24515 23644..24147 + 504 WP_024188971.1 hypothetical protein -
HXS63_RS24520 24151..25380 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
HXS63_RS24525 25457..25750 + 294 WP_000134733.1 LuxR family transcriptional regulator -
HXS63_RS24530 25747..25962 + 216 WP_000760375.1 EexN family lipoprotein -
HXS63_RS24535 26139..26315 + 177 WP_000660496.1 hypothetical protein -
HXS63_RS24540 26318..26938 + 621 WP_074169730.1 lytic transglycosylase domain-containing protein -
HXS63_RS24545 27006..27278 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32452 32452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T36987 WP_000520549.1 NZ_AP023229:22691-22939 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T36987 NZ_AP023229:22691-22939 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT36987 WP_000681613.1 NZ_AP023229:22936-23283 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT36987 NZ_AP023229:22936-23283 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References