Detailed information of TA system TA036986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 20734..21199
• Replicon: plasmid pYJ3-b
• Accession: NZ_AP023229
• Organism: Escherichia coli strain YJ3

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: -
• Locus tag: HXS63_RS24485
• Protein ID: WP_086621303.1
• Coordinates: 20734..20985 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: HXS63_RS24490
• Protein ID: WP_000801440.1
• Coordinates: 20972..21199 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS63_RS24460	16214..16741	-	528	WP_000577044.1	DUF2726 domain-containing protein	-
HXS63_RS24465	16877..17281	-	405	WP_012421203.1	hypothetical protein	-
HXS63_RS24470	17294..18160	-	867	WP_050427389.1	replication initiation protein	-
HXS63_RS24475	19575..20090	+	516	WP_001025395.1	J domain-containing protein	-
HXS63_RS24480	20195..20737	+	543	WP_001420167.1	hypothetical protein	-
HXS63_RS24485	20734..20985	+	252	WP_086621303.1	hypothetical protein	Toxin
HXS63_RS24490	20972..21199	+	228	WP_000801440.1	hypothetical protein	Antitoxin
HXS63_RS24495	21642..21941	+	300	WP_142284800.1	hypothetical protein	-
HXS63_RS24500	22036..22386	+	351	WP_063848416.1	hypothetical protein	-
HXS63_RS24505	22691..22939	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
HXS63_RS24510	22936..23283	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
HXS63_RS24515	23644..24147	+	504	WP_024188971.1	hypothetical protein	-
HXS63_RS24520	24151..25380	+	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
HXS63_RS24525	25457..25750	+	294	WP_000134733.1	LuxR family transcriptional regulator	-
HXS63_RS24530	25747..25962	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32452	32452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9737.10 Da
• Isoelectric Point: 8.9187

>T36986 WP_086621303.1 NZ_AP023229:20734-20985 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCSGLREDDEAPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T36986 NZ_AP023229:20734-20985 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTTCCGGATTAAGGGAAGACGATGAGGCGCCTGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT36986 WP_000801440.1 NZ_AP023229:20972-21199 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT36986 NZ_AP023229:20972-21199 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACATGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A5Y6U8W9